Fabrication and in vitro evaluation of the collagen/hyaluronic acid PEM coating crosslinked with functionalized RGD peptide on titanium

被引：41

作者：

Huang, Ying ^{[1
]}

Luo, Qiaojie ^{[1
]}

Li, Xiaodong ^{[1
]}

Zhang, Feng ^{[1
]}

Zhao, Shifang ^{[1
]}

机构：

[1] Zhejiang Univ, Coll Med, Affiliated Stomatol Hosp, Dept Oral & Maxillofacial Surg, Hangzhou 310006, Zhejiang, Peoples R China

来源：

ACTA BIOMATERIALIA | 2012年 / 8卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Surface modification; Functionalized RGD peptide; Disulfide; Layer by layer technique; Preosteoblasts; BONE-FORMATION; DENTAL IMPLANTS; CELL-ADHESION; POLYELECTROLYTE MULTILAYERS; PROGRESSIVE DEVELOPMENT; OSTEOBLAST PHENOTYPE; DIFFERENTIATION; SURFACES; COLLAGEN; IMMOBILIZATION;

D O I：

10.1016/j.actbio.2011.10.020

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

Surface modification of titanium (Ti) using biomolecules has attracted much attention recently. In this study, a new strategy has been employed to construct a stable and bioactive coating on Ti. To this end, a derivative of hyaluronic acid (HA), i.e. HA-GRGDSPC-(SH), was synthesized. The disulfide-crosslinked Arg-Gly-Asp (RGD)-containing collagen/hyaluronic acid polyelectrolyte membrane (REM) coating was then fabricated on Ti through the alternate deposition of collagen and HA-GRGDSPC-(SH) with five assembly cycles and subsequent crosslinking via converting free sulphydryl groups into disulfide linkages (RGD-CHC-Ti group). The assembly processes for PEM coating and the physicochemical properties of the coating were carefully characterized. The stability of PEM coating in phosphate-buffered saline solution could be adjusted by the crosslinking degree, while its degradation behaviors in the presence of glutathione were glutathione concentration dependent. The adhesion and proliferation of MC3T3-E1 cells were significantly enhanced in the RGD-CHC-Ti group. Up-regulated bone specific genes, enhanced alkaline phosphatase activity and osteocalcin production, the increased areas of mineralization were also observed in the RGD-CHC-Ti group. These results indicate that the strategy employed herein may function as an effective way to construct stable, RGD-containing bioactive coatings on Ti. (C) 2011 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

引用

页码：866 / 877

页数：12

共 48 条

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