Perfusion-decellularized matrix: using nature's platform to engineer a bioartificial heart

被引:1879
作者
Ott, Harald C. [2 ]
Matthiesen, Thomas S. [1 ]
Goh, Saik-Kia [1 ]
Black, Lauren D. [3 ]
Kren, Stefan M. [1 ]
Netoff, Theoden I. [3 ]
Taylor, Doris A. [1 ,4 ]
机构
[1] Univ Minnesota, Ctr Cardiovasc Repair, Minneapolis, MN 55455 USA
[2] Harvard Univ, Sch Med, Massachusetts Gen Hosp, Boston, MA 02114 USA
[3] Univ Minnesota, Dept Biomed Engn, Minneapolis, MN 55455 USA
[4] Univ Minnesota, Dept Integrat Biol & Physiol, Minneapolis, MN 55455 USA
关键词
D O I
10.1038/nm1684
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
About 3,000 individuals in the United States are awaiting a donor heart; worldwide, 22 million individuals are living with heart failure. A bioartificial heart is a theoretical alternative to transplantation or mechanical left ventricular support. Generating a bioartificial heart requires engineering of cardiac architecture, appropriate cellular constituents and pump function. We decellularized hearts by coronary perfusion with detergents, preserved the underlying extracellular matrix, and produced an acellular, perfusable vascular architecture, competent acellular valves and intact chamber geometry. To mimic cardiac cell composition, we reseeded these constructs with cardiac or endothelial cells. To establish function, we maintained eight constructs for up to 28 d by coronary perfusion in a bioreactor that simulated cardiac physiology. By day 4, we observed macroscopic contractions. By day 8, under physiological load and electrical stimulation, constructs could generate pump function (equivalent to about 2% of adult or 25% of 16-week fetal heart function) in a modified working heart preparation.
引用
收藏
页码:213 / 221
页数:9
相关论文
共 26 条
[1]   DAMAGE OF PORCINE AORTIC-VALVE TISSUE CAUSED BY THE SURFACTANT SODIUMDODECYLSULFATE [J].
BODNAR, E ;
OLSEN, EGJ ;
FLORIO, R ;
DOBRIN, J .
THORACIC AND CARDIOVASCULAR SURGEON, 1986, 34 (02) :82-85
[2]   Process development of an acellular dermal matrix (ADM) for biomedical applications [J].
Chen, RN ;
Ho, HO ;
Tsai, YT ;
Sheu, MT .
BIOMATERIALS, 2004, 25 (13) :2679-2686
[3]   DEVELOPMENT OF A PERICARDIAL ACELLULAR MATRIX BIOMATERIAL - BIOCHEMICAL AND MECHANICAL EFFECTS OF CELL EXTRACTION [J].
COURTMAN, DW ;
PEREIRA, CA ;
KASHEF, V ;
MCCOMB, D ;
LEE, JM ;
WILSON, GJ .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, 1994, 28 (06) :655-666
[4]   Eleven years' experience with the Biocor stentless aortic bioprosthesis:: clinical and hemodynamic follow-up with long-term relative survival rate [J].
Dellgren, G ;
Eriksson, MJ ;
Brodin, LÅ ;
Rådegran, K .
EUROPEAN JOURNAL OF CARDIO-THORACIC SURGERY, 2002, 22 (06) :912-921
[5]   Engineering myocardial tissue [J].
Eschenhagen, T ;
Zimmermann, WH .
CIRCULATION RESEARCH, 2005, 97 (12) :1220-1231
[6]   Pulsatile cardiac tissue grafts using a novel three-dimensional cell sheet manipulation technique functionally integrates with the host heart, in vivo [J].
Furuta, A ;
Miyoshi, S ;
Itabashi, Y ;
Shimizu, T ;
Kira, S ;
Hayakawa, K ;
Nishiyama, N ;
Tanimoto, K ;
Hagiwara, Y ;
Satoh, T ;
Fukuda, K ;
Okano, T ;
Ogawa, S .
CIRCULATION RESEARCH, 2006, 98 (05) :705-712
[7]   Biophysical regulation during cardiac development and application to tissue engineering [J].
Gerecht-Nir, S ;
Radisic, M ;
Park, H ;
Cannizzaro, C ;
Boublik, J ;
Langer, R ;
Vunjak-Novakovic, G .
INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY, 2006, 50 (2-3) :233-243
[8]   Decellularization of tissues and organs [J].
Gilbert, TW ;
Sellaro, TL ;
Badylak, SF .
BIOMATERIALS, 2006, 27 (19) :3675-3683
[9]   Mechanical and structural properties of a novel hybrid heart valve scaffold for tissue engineering [J].
Grabow, N ;
Schmohl, K ;
Khosravi, A ;
Philipp, M ;
Scharfschwerdt, M ;
Graf, B ;
Stamm, C ;
Haubold, A ;
Schmitz, KP ;
Steinhoff, G .
ARTIFICIAL ORGANS, 2004, 28 (11) :971-979
[10]   Intracardiac pressures in the human fetus [J].
Johnson, P ;
Maxwell, DJ ;
Tynan, MJ ;
Allan, LD .
HEART, 2000, 84 (01) :59-63