In vitro alleviation of heavy metal-induced enzyme inhibition by proline

被引：154

作者：

Sharma, SS ^{[1
]}

Schat, H ^{[1
]}

Vooijs, R ^{[1
]}

机构：

[1] Free Univ Amsterdam, Fac Biol, Dept Ecol & Ecotoxicol, NL-1081 HV Amsterdam, Netherlands

来源：

PHYTOCHEMISTRY | 1998年 / 49卷 / 06期

关键词：

cadmium; zinc; glucose-6-phosphate dehydrogenase; heavy metal stress; nitrate reductase; proline;

D O I：

10.1016/S0031-9422(98)00282-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Accumulation of proline in response to toxic heavy metal exposure seems to be wide-spread among plants. To elucidate the role for proline in plant responses to heavy metal stress, we studied the effect of proline on Cd-induced and Zn-induced inhibition of glucose-6-phosphate dehydrogenase (G-6-PDH; EC 1.1.1.49) and nitrate reductase (NR; EC 1.6.6.2) in vitro. Proline appeared to protect both enzymes against Zn and, though less effectively, against Cd. Measurements with a Cd2+-specific electrode strongly suggested that this protection was based on a reduction of the free metal ion activity in the assay buffer, due to the formation of metal-proline complexes. There were no indications of any significant role for proline-water or proline-protein interactions. The significance of these findings with regard to heavy metal-induced proline accumulation in viva is discussed. (C) 1998 Elsevier Science Ltd. All rights reserved.

引用

页码：1531 / 1535

页数：5

共 36 条

[11] PHYTOCHELATINS IN CADMIUM-SENSITIVE AND CADMIUM-TOLERANT SILENE VULGARIS - CHAIN-LENGTH DISTRIBUTION AND SULFIDE INCORPORATION [J].

DEKNECHT, JA ;

VANDILLEN, M ;

KOEVOETS, PLM ;

SCHAT, H ;

VERKLEIJ, JAC ;

ERNST, WHO .

PLANT PHYSIOLOGY, 1994, 104 (01) :255-261

[12] PHYTOCHELATINS, A CLASS OF HEAVY-METAL-BINDING PEPTIDES FROM PLANTS, ARE FUNCTIONALLY ANALOGOUS TO METALLOTHIONEINS [J].

GRILL, E ;

WINNACKER, EL ;

ZENK, MH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (02) :439-443

[13] OXYGEN-TOXICITY, OXYGEN RADICALS, TRANSITION-METALS AND DISEASE [J].

HALLIWELL, B ;

GUTTERIDGE, JMC .

BIOCHEMICAL JOURNAL, 1984, 219 (01) :1-14

[14] STABILIZATION AND INACTIVATION OF BIOLOGICAL MEMBRANES DURING FREEZING IN PRESENCE OF AMINO ACIDS [J].

HEBER, U ;

TYANKOVA, L ;

SANTARIUS, KA .

BIOCHIMICA ET BIOPHYSICA ACTA, 1971, 241 (02) :578-+

[15] EFFECT OF EXCESS LEAD, CADMIUM, COPPER, AND ZINC ON WATER RELATIONS IN SUNFLOWER [J].

KASTORI, R ;

PETROVIC, M ;

PETROVIC, N .

JOURNAL OF PLANT NUTRITION, 1992, 15 (11) :2427-2439

[16] OVEREXPRESSION OF DELTA-PYRROLINE-5-CARBOXYLATE SYNTHETASE INCREASES PROLINE PRODUCTION AND CONFERS OSMOTOLERANCE IN TRANSGENIC PLANTS [J].

KISHOR, PBK ;

HONG, ZL ;

MIAO, GH ;

HU, CAA ;

VERMA, DPS .

PLANT PHYSIOLOGY, 1995, 108 (04) :1387-1394

[17] PHYTOCHELATINS PROTECT PLANT ENZYMES FROM HEAVY-METAL POISONING [J].

KNEER, R ;

ZENK, MH .

PHYTOCHEMISTRY, 1992, 31 (08) :2663-2667

[18] PROTECTION OF PYRUVATE, PI DIKINASE FROM MAIZE AGAINST COLD LABILITY BY COMPATIBLE SOLUTES [J].

KRALL, JP ;

EDWARDS, GE ;

ANDREO, CS .

PLANT PHYSIOLOGY, 1989, 89 (01) :280-285

[19] COPPER TOLERANCE IN SILENE-CUCUBALUS - SUBCELLULAR-DISTRIBUTION OF COPPER AND ITS EFFECTS ON CHLOROPLASTS AND PLASTOCYANIN SYNTHESIS [J].

LOLKEMA, PC ;

VOOIJS, R .

PLANTA, 1986, 167 (01) :30-36

[20]

Martell A.E., 1982, CRITICAL STABILITY C

← 1 2 3 4 →