Nonequivalent nuclear location of immunoglobulin alleles in B lymphocytes

被引:168
作者
Skok, JA
Brown, KE
Azuara, V
Caparros, ML
Baxter, J
Takacs, K
Dillon, N
Gray, D
Perry, RP
Merkenschlager, M
Fisher, AG
机构
[1] Hammersmith Hosp, Imperial Coll Sch Med, MRC, Clin Sci Ctr,Lymphocyte Dev Grp, London W12 0NN, England
[2] Hammersmith Hosp, Imperial Coll Sch Med, MRC, Clin Sci Ctr,Gene Regulat & Chromatin Grp, London W12 0NN, England
[3] Univ Edinburgh, Inst Cell Anim & Populat Biol, Ashworth Labs, Edinburgh EH9 3JT, Midlothian, Scotland
[4] Fox Chase Canc Ctr, Inst Canc Res, Philadelphia, PA 19111 USA
基金
英国惠康基金; 英国医学研究理事会;
关键词
D O I
10.1038/ni0901-848
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Individual B lymphocytes normally express immunoglobulin (Ig) proteins derived from single Ig heavy chain (H) and light chain (L) alleles. Allelic exclusion ensures monoallelic expression of Ig genes by each B cell to maintain single receptor specificity. Here we provide evidence that at later stages of B cell development, additional mechanisms may contribute to prioritizing expression of single IgH and IgL alleles. Fluorescent in situ hybridization analysis of primary splenic B cells isolated from normal and genetically manipulated mice showed that endogenous IgH, kappa and lambda alleles localized to different subnuclear environments after activation and had differential expression patterns. However, this differential recruitment and expression of Ig alleles was not typically seen among transformed B cell lines. These data raise the possibility that epigenetic factors help maintain the monoallelic expression of Ig.
引用
收藏
页码:848 / 854
页数:7
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