Response of enzyme activities to nitrogen addition in forest floors of different C-to-N ratios

被引:61
作者
Michel, K [1 ]
Matzner, E [1 ]
机构
[1] Univ Bayreuth, Bayreuth Inst Terr Ecosyst Res, Dept Soil Energy, D-95440 Bayreuth, Germany
关键词
enzymes; forest floor; Oa material; nitrogen addition;
D O I
10.1007/s00374-003-0622-5
中图分类号
S15 [土壤学];
学科分类号
0903 ; 090301 ;
摘要
The effect of different inputs of mineral N on several enzyme activities involved in the C and N cycles was investigated using Oa material of forest floors from four Norway spruce [Picea abies (L.) Karst.] sites with different C-to-N ratios. The samples from each site were treated with five different concentrations of mineral N (as liquid NH4NO3). All samples were incubated aerobically for 15-20 weeks at 15degreesC and at field capacity. Respiration was measured weekly. At the end of the incubation period, four enzyme activities (endoglucanase, beta-glucosidase, polyphenol oxidase and beta-glucosaminidase) and microbial biomass were determined. Endoglucanase activity was increased and beta-glucosidase activity was decreased by N additions only in Oa material having a wide C-to-N ratio. In N-supplemented samples of low C-to-N ratio, increased polyphenol oxidase activities were often detected as a consequence of N addition. beta-Glucosaminidase activity responded positively to mineral N additions, particularly in Oa samples with low internal N concentration. The results of the present study indicate that the effects of N additions on enzymatic activities of organic matter in late stages of decomposition are related to the C-to-N ratio. Increasing inputs of mineral N to spruce ecosystems may especially affect C-hydrolyzing enzyme activities in soils with wide C-to-N ratio leading to an incomplete degradation of cellulose and thus reduced C availability to micro-organisms.
引用
收藏
页码:102 / 109
页数:8
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