Angiotensin I-Converting Enzyme Inhibitory Activity of Gelatin Hydrolysates and Identification of Bioactive Peptides

被引：56

作者：

Herregods, Griet ^{[1
,2
]}

Van Camp, John ^{[2
]}

Morel, Nicole ^{[3
]}

Ghesquiere, Bart ^{[4
,5
]}

Gevaert, Kris ^{[4
,5
]}

Vercruysse, Lieselot ^{[1
,2
]}

Dierckx, Stephan ^{[6
]}

Quanten, Erwin ^{[6
]}

Smagghe, Guy ^{[1
]}

机构：

[1] Univ Ghent, Dept Crop Protect, Fac Biosci Engn, B-9000 Ghent, Belgium

[2] Univ Ghent, Dept Food Safety & Food Qual, Fac Biosci Engn, B-9000 Ghent, Belgium

[3] Catholic Univ Louvain, Fac Med, Lab Cellular Physiol, B-1200 Brussels, Belgium

[4] VIB, Dept Med Prot Res, B-9000 Ghent, Belgium

[5] Univ Ghent, Dept Biochem, B-9000 Ghent, Belgium

[6] Tessenderlo Grp, PB Gelatins, B-1800 Vilvoorde, Belgium

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2011年 / 59卷 / 02期

关键词：

Gelatin hydrolysate; antihypertensive peptides; angiotensin I-converting enzyme (ACE); blood pressure; gastrointestinal and mucosal digestion; spontaneously hypertensive rats; aortic rings; HYPERTENSIVE-RATS; ORAL INGESTION; BLOOD-PRESSURE; WHEY-PROTEIN; FOOD; CELLS; VITRO; SKIN;

D O I：

10.1021/jf1037823

中图分类号：

S [农业科学];

学科分类号：

082806 [农业信息与电气工程];

摘要：

In this project we report on the angiotensin I-converting enzyme (ACE)-inhibitory activity of a bovine gelatin hydrolysate (Bh2) that was submitted to further hydrolysis by different enzymes. The thermolysin hydrolysate (Bh2t) showed the highest in vitro ACE inhibitory activity, and interestingly a marked in vivo blood pressure-lowering effect was demonstrated in spontaneously hypertensive rats (SHR). In contrast, Bh2 showed no effect in SHR, confirming the need for the extra thermolysin hydrolysis. Hence, an angiotensin I-evoked contractile response in isolated rat aortic rings was inhibited by Bh2t, but not by Bh2, suggesting ACE inhibition as the underlying antihypertensive mechanism for Bh2t. Using mass spectrometry, seven small peptides, AG, AGP, VGP, PY, QY, DY and IV or LY or HO-PY were identified in Bh2t. As these peptides showed ACE inhibitory activity and were more prominent in Bh2t than in Bh2, the current data provide evidence that these contribute to the antihypertensive effect of Bh2t.

引用

页码：552 / 558

页数：7

共 27 条

[1]

Purification and characterization of angiotensin I converting enzyme (ACE) inhibitory peptides from Alaska pollack (Theragra chalcogramma) skin [J].