A sequence motif responsible for ER export and surface expression of Kir2.0 inward rectifier K+ channels

被引:87
作者
Stockklausner, C [1 ]
Ludwig, J [1 ]
Ruppersberg, JP [1 ]
Klöcker, N [1 ]
机构
[1] Univ Tubingen, Dept Physiol 2, D-72074 Tubingen, Germany
关键词
endoplasmic reticulum; Golgi; potassium channel; PSD-95;
D O I
10.1016/S0014-5793(01)02286-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Integral membrane proteins are sorted va the secretory pathway. It was proposed that this pathway is nonselective provided that the cargo protein is properly assembled and lacks an endoplasmic reticulum (ER) retention signal, However, recent experimental evidence suggests that efficient export of proteins from the ER to the Golgi complex is not simply a default pathway. Here we demonstrate a novel sequence motif (FsYENEV) in the cytoplasmic C-terminus of mammalian inward rectifier potassium (Kir) channels which determines ER export, This motif is found to be both necessary and sufficient for efficient export from the ER that eventually leads to efficient surface expression of Kir2.1 channels. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V, All rights reserved,
引用
收藏
页码:129 / 133
页数:5
相关论文
共 23 条
[1]   VESICULAR STOMATITIS-VIRUS GLYCOPROTEIN IS SORTED AND CONCENTRATED DURING EXPORT FROM THE ENDOPLASMIC-RETICULUM [J].
BALCH, WE ;
MCCAFFERY, JM ;
PLUTNER, H ;
FARQUHAR, MG .
CELL, 1994, 76 (05) :841-852
[2]   COPI- and COPII-coated vesicles bud directly from the endoplasmic reticulum in yeast [J].
Bednarek, SY ;
Ravazzola, M ;
Hosobuchi, M ;
Amherdt, M ;
Perrelet, A ;
Schekman, R ;
Orci, L .
CELL, 1995, 83 (07) :1183-1196
[3]   gp25L/emp24/p24 protein family members of the cis-Golgi network bind both COP I and II coatomer [J].
Dominguez, M ;
Dejgaard, K ;
Füllekrug, J ;
Dahan, S ;
Fazel, A ;
Paccaud, JP ;
Thomas, DY ;
Bergeron, JJM ;
Nilsson, T .
JOURNAL OF CELL BIOLOGY, 1998, 140 (04) :751-765
[4]   Heterooligomeric assembly of inward-rectifier K+ channels from subunits of different subfamilies: K(ir)2.1 (IRK1) and K(ir)4.1 (BIR10) [J].
Fakler, B ;
Bond, CT ;
Adelman, JP ;
Ruppersberg, JP .
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1996, 433 (1-2) :77-83
[5]   Bimodal interaction of coatomer with the p24 family of putative cargo receptors [J].
Fiedler, K ;
Veit, M ;
Stamnes, MA ;
Rothman, JE .
SCIENCE, 1996, 273 (5280) :1396-1399
[6]  
Hibino H, 1997, J NEUROSCI, V17, P4711
[7]   Clustering and enhanced activity of an inwardly rectifying potassium channel, Kir4.1, by an anchoring protein, PSD-95/SAP90 [J].
Horio, Y ;
Hibino, H ;
Inanobe, A ;
Yamada, M ;
Ishii, M ;
Tada, Y ;
Satoh, E ;
Hata, Y ;
Takai, Y ;
Kurachi, Y .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (20) :12885-12888
[8]  
Ishii M, 1997, J NEUROSCI, V17, P7725
[9]   The recycling of ERGIC-53 in the early secretory pathway - ERGIC-53 carries a cytosolic endoplasmic reticulum exit determinant interacting with COPII [J].
Kappeler, F ;
Klopfenstein, DRC ;
Foguet, M ;
Paccaud, JP ;
Hauri, HP .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (50) :31801-31808
[10]   Transport between ER and Golgi [J].
Klumperman, J .
CURRENT OPINION IN CELL BIOLOGY, 2000, 12 (04) :445-449