Molecular markers for the scab resistance (Vf) region in apple

被引：37

作者：

Hemmat, M ^{[1
]}

Weeden, NF

Aldwinckle, HS

Brown, SK

机构：

[1] Cornell Univ, New York State Agr Expt Stn, Dept Hort Sci, Geneva, NY 14456 USA

[2] Cornell Univ, New York State Agr Expt Stn, Dept Plant Pathol, Geneva, NY 14456 USA

来源：

JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE | 1998年 / 123卷 / 06期

关键词：

Malus; markers; STS; multiplexing;

D O I：

10.21273/JASHS.123.6.992

中图分类号：

S6 [园艺];

学科分类号：

0902 ;

摘要：

Bulked segregant analysis was used to identify RAPD markers that display tight linkage to the V-f gene in apple (Malus sp.) that confers resistance to five races of apple scab [Venturia inaequalis (Cke.) Wint.]. We identified several new RAPD markers linked to V-f. The most tightly linked marker in the test population, S5(2500), was cloned and sequenced. A linkage map of the V-f region was developed using these markers, RAPD markers previously described by other laboratories, and the isozyme locus Pgm-1, An assay was developed for V-f by multiplexing the two markers closely flanking the V-f locus. This assay has a theoretical 'escape' value (discarding a resistant plant) of 3% and an error rate (selection of a susceptible plant) of 0.02%.

引用

页码：992 / 996

页数：5

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