Nucellar-cell-specific expression of a lipid transfer protein gene in barley (Hordeum vulgare L.)

被引:9
作者
Chen, F [1 ]
Foolad, MR [1 ]
机构
[1] Penn State Univ, Dept Hort, University Pk, PA 16802 USA
关键词
plant lipid transfer protein; barley; ovary; nucellus; gene expression;
D O I
10.1007/s002990050601
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Plant lipid transfer proteins (LTPs) are thought to participate in many aspects of cell growth and development. We have identified a novel LTP gene (LTP-ne) from barley (Hordeum vulgare) through differential screening of an ovary cDNA library. LTP-ne was mapped to the distal region of the long arm of barley chromosome 4, distinct from the genomic location of other known LTPs. Sequence comparison indicated that LTP-ne was more closely related to the 7-kDa LTPs than to the 9-kDa LTPs in barley. In Northern hybridization, LTP-ne transcripts were detected in ovary, but not in other tissues, and were most abundant 3-4 days after pollination. Furthermore, RNA in situ hybridization showed that within the ovary, the LTP-ne gene was specifically expressed in the outer cell layer of the nucellus. These results showed that the newly identified barley LTP was ovular cell specific and regulated by pollination/fertilization.
引用
收藏
页码:445 / 450
页数:6
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