An Alpha-Catulin Homologue Controls Neuromuscular Function through Localization of the Dystrophin Complex and BK Channels in Caenorhabditis elegans

被引:27
作者
Abraham, Linu S. [1 ]
Oh, Hyun J. [1 ]
Sancar, Feyza [2 ]
Richmond, Janet E. [2 ]
Kim, Hongkyun [1 ]
机构
[1] Rosalind Franklin Univ Med & Sci, Chicago Med Sch, Dept Cell Biol & Anat, N Chicago, IL USA
[2] Univ Illinois, Dept Biol Sci, Chicago, IL 60680 USA
关键词
CA2+-ACTIVATED K+ CHANNELS; POTASSIUM CHANNEL; C-ELEGANS; PYRAMIDAL NEURONS; ION CHANNELS; DYSTROBREVIN; VINCULIN; BINDING; CHROMAFFIN; EXPRESSION;
D O I
10.1371/journal.pgen.1001077
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The large conductance, voltage- and calcium-dependent potassium (BK) channel serves as a major negative feedback regulator of calcium-mediated physiological processes and has been implicated in muscle dysfunction and neurological disorders. In addition to membrane depolarization, activation of the BK channel requires a rise in cytosolic calcium. Localization of the BK channel near calcium channels is therefore critical for its function. In a genetic screen designed to isolate novel regulators of the Caenorhabditis elegans BK channel, SLO-1, we identified ctn-1, which encodes an alpha-catulin homologue with homology to the cytoskeletal proteins a-catenin and vinculin. ctn-1 mutants resemble slo-1 loss-of-function mutants, as well as mutants with a compromised dystrophin complex. We determined that CTN-1 uses two distinct mechanisms to localize SLO-1 in muscles and neurons. In muscles, CTN-1 utilizes the dystrophin complex to localize SLO-1 channels near L-type calcium channels. In neurons, CTN-1 is involved in localizing SLO-1 to a specific domain independent of the dystrophin complex. Our results demonstrate that CTN-1 ensures the localization of SLO-1 within calcium nanodomains, thereby playing a crucial role in muscles and neurons.
引用
收藏
页数:13
相关论文
共 48 条
  • [31] Direct interaction between BKCa potassium channel and microtubule-associated protein 1A
    Park, SM
    Liu, GX
    Kubal, A
    Fury, M
    Cao, LX
    Marx, SO
    [J]. FEBS LETTERS, 2004, 570 (1-3): : 143 - 148
  • [32] Genetic analysis of crawling and swimming locomotory patterns in C. elegans
    Pierce-Shimomura, Jonathan T.
    Chen, Beth L.
    Mun, James J.
    Ho, Raymond
    Sarkis, Raman
    McIntire, Steven L.
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (52) : 20982 - 20987
  • [33] BK channel activation by brief depolarizations requires Ca2+ influx through L- and Q-type Ca2+ channels in rat chromaffin cells
    Prakriya, M
    Lingle, CJ
    [J]. JOURNAL OF NEUROPHYSIOLOGY, 1999, 81 (05) : 2267 - 2278
  • [34] Richardson J. J., 2006, ELECTROPHYSIOLOGICAL, P1, DOI [10.1096/fj.06-6264fje, DOI 10.1096/FJ.06-6264FJE]
  • [35] Dystrobrevin and dystrophin: An interaction through coiled-coil motifs
    SadouletPuccio, HM
    Rajala, M
    Kunkel, LM
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (23) : 12413 - 12418
  • [36] Presynaptic CaV2 calcium channel traffic requires CALF-1 and the α2δ subunit UNC-36
    Saheki, Yasunori
    Bargmann, Cornelia I.
    [J]. NATURE NEUROSCIENCE, 2009, 12 (10) : 1257 - U73
  • [37] Immunolocalization of BK channels in hippocampal pyramidal neurons
    Sailer, Claudia A.
    Kaufmann, Walter A.
    Kogler, Michaela
    Chen, Lie
    Sausbier, Ulrike
    Ottersen, Ole Petter
    Ruth, Peter
    Shipston, Michael J.
    Knaus, Hans-Guenther
    [J]. EUROPEAN JOURNAL OF NEUROSCIENCE, 2006, 24 (02) : 442 - 454
  • [38] High-conductance potassium channels of the SLO family
    Salkoff, Lawrence
    Butler, Alice
    Ferreira, Gonzalo
    Santi, Celia
    Wei, Aguan
    [J]. NATURE REVIEWS NEUROSCIENCE, 2006, 7 (12) : 921 - 931
  • [39] Slob, a novel protein that interacts with the slowpoke calcium-dependent potassium channel
    Schopperle, WM
    Holmqvist, MH
    Zhou, Y
    Wang, J
    Wang, Z
    Griffith, LC
    Keselman, I
    Kusinitz, F
    Dagan, D
    Levitan, IB
    [J]. NEURON, 1998, 20 (03) : 565 - 573
  • [40] Shioi G, 2001, GENETICS, V157, P1611