Human VPS34 and p150 are rab7 interacting partners

被引：138

作者：

Stein, MP ^{[1
]}

Feng, Y ^{[1
]}

Cooper, KL ^{[1
]}

Welford, AM ^{[1
]}

Wandinger-Ness, A ^{[1
]}

机构：

[1] Univ New Mexico, Sch Med, Dept Pathol, Mol Trafficking Lab, Albuquerque, NM 87131 USA

来源：

TRAFFIC | 2003年 / 4卷 / 11期

关键词：

endocytosis; membrane trafficking; phosphatidylinositol 3 '-kinase; phosphoinositides; vesicular transport;

D O I：

10.1034/j.1600-0854.2003.00133.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Regulation of membrane trafficking requires the concerted actions of rab proteins, their effectors and several phosphatidylinositol 3'-kinases. Rab7 is required for late endosomal transport and here we establish that the phosphatidylinositol 3'-kinase hVPS34 and its adaptor protein p150 are rab7 interacting partners. The hVPS34/p150 complex colocalized with rab7 on late endosomes and hVPS34 activity was dependent on nucleotide cycling of rab7. In addition, total cellular phosphatidylinositol 3'-phosphate levels were modulated by rab7 expression, suggesting that rab7 activation impacted kinase cycling to early endosomes. The data identify rab7 as an important regulator of late endosomal hVPS34 function and link rab7 to the regulation of phosphatidylinositol 3'-kinase cycling between early and late endosomes.

引用

页码：754 / 771

页数：18

共 47 条

[1] Visualization of Rab9-mediated vesicle transport from endosomes to the trans-Golgi in living cells [J].

Barbero, P ;

Bittova, L ;

Pfeffer, SR .

JOURNAL OF CELL BIOLOGY, 2002, 156 (03) :511-518

[2] Rab7: A key to lysosome biogenesis [J].

Bucci, C ;

Thomsen, P ;

Nicoziani, P ;

McCarthy, J ;

van Deurs, B .

MOLECULAR BIOLOGY OF THE CELL, 2000, 11 (02) :467-480

[3] Expression analysis and chromosomal assignment of PRA1 and RILP genes [J].

Bucci, C ;

De Gregorio, L ;

Bruni, CB .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2001, 286 (04) :815-819

[4] SPECIFIC INTERACTIONS OF MSS4 WITH MEMBERS OF THE RAB GTPASE SUBFAMILY [J].

BURTON, JL ;

BURNS, ME ;

GATTI, E ;

AUGUSTINE, GJ ;

DECAMILLI, P .

EMBO JOURNAL, 1994, 13 (23) :5547-5558

[5] Rab-interacting lysosomal protein (RILP): the Rab7 effector required for transport to lysosomes [J].

Cantalupo, G ;

Alifano, P ;

Roberti, V ;

Bruni, CB ;

Bucci, C .

EMBO JOURNAL, 2001, 20 (04) :683-693

[6] Phosphatidylinositol-3-OH kinases are Rab5 effectors [J].

Christoforidis, S ;

Miaczynska, M ;

Ashman, K ;

Wilm, M ;

Zhao, LY ;

Yip, SC ;

Waterfield, MD ;

Backer, JM ;

Zerial, M .

NATURE CELL BIOLOGY, 1999, 1 (04) :249-252

[7] The Rab5 effector EEA1 is a core component of endosome docking [J].

Christoforidis, S ;

McBride, HM ;

Burgoyne, RD ;

Zerial, M .

NATURE, 1999, 397 (6720) :621-625

[8] Divalent Rab effectors regulate the sub-compartmental organization and sorting of early endosomes [J].

De Renzis, S ;

Sönnichsen, B ;

Zerial, M .

NATURE CELL BIOLOGY, 2002, 4 (02) :124-133

[9] RAB-7 - AN IMPORTANT REGULATOR OF LATE ENDOCYTIC MEMBRANE TRAFFIC [J].

FENG, Y ;

PRESS, B ;

WANDINGERNESS, A .

JOURNAL OF CELL BIOLOGY, 1995, 131 (06) :1435-1452

[10]

Feng Y, 2001, METHOD ENZYMOL, V329, P175

← 1 2 3 4 5 →