Real-time detection of hyperosmotic stress response in optically trapped single yeast cells using Raman microspectroscopy

被引：69

作者：

Singh, GP

Creely, CM

Volpe, G

Grötsch, H

Petrov, D

机构：

[1] ICFO, Inst Ciencies Foton, Barcelona 08034, Spain

[2] IBMB, Inst Biol Mol Barcelona, Barcelona 08034, Spain

[3] ICREA, Inst Catalana Recerca & Estudis Avancats, Barcelona 08015, Spain

来源：

ANALYTICAL CHEMISTRY | 2005年 / 77卷 / 08期

关键词：

D O I：

10.1021/ac048359j

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Living cells survive environmentally stressful conditions by initiating a stress response. We monitored changes in the Raman spectra of optically trapped Saccharomyces cerevisiae yeast cell under normal, heat-treated, and hyperosmotic stress conditions. It is shown that when glucose was used to exert hyperosmotic stress, two chemical substances-glycerol and ethanol-can be monitored in real time in a single cell.

引用

收藏

页码：2564 / 2568

页数：5

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