Biodegradable and thermo-sensitive chitosan-g-poly(N-vinylcaprolactam) nanoparticles as a 5-fluorouracil carrier

被引:133
作者
Rejinold, N. Sanoj [1 ]
Chennazhi, K. P. [1 ]
Nair, S. V. [1 ]
Tamura, H. [2 ]
Jayakumar, R. [1 ]
机构
[1] Amrita Vishwa Vidyapeetham Univ, Amrita Ctr Nanosci & Mol Med, Amrita Inst Med Sci & Res Ctr, Kochi 682041, India
[2] Kansai Univ, Fac Chem Mat & Bioengn, Osaka 5648680, Japan
关键词
Thermo responsive graft co polymeric nanoparticles; LCST; Biomaterials; Cancer drug delivery; TISSUE-ENGINEERING APPLICATIONS; COMPOSITE SCAFFOLDS; CHITIN MEMBRANES; DRUG-DELIVERY; CHITOSAN; CANCER; 5-FU;
D O I
10.1016/j.carbpol.2010.08.052
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
We developed a nanoformulation of 5-FU (5-fluorouracil) with biodegradable thermo-responsive chitosan-g-poly(N-vinylcaprolactam) biopolymer composite for its delivery to cancer cells The novel thermo-responsive graft co-polymeric nanoparticles (TRC-NPs) were prepared by ionic cross-linking method which showed a lower critical solution temperature (LCST) at 38 degrees C The 5-FU drug was incorporated into the carrier using cross-linking reaction The in vitro drug release showed prominent release above LCST Cytotoxicity assay showed TRC-NPs in the concentration range of 100-100 mu g/ml are nontoxic to an array of cell lines The drug-loaded nanoparticles showed comparatively higher toxicity to cancer cells while they are less toxic to normal cells The cell uptake of the 5-FU loaded thermo-responsive graft co-polymeric nanoparticles (5-FU-TRC-NP5) was confirmed from green fluorescence inside cells by rhodamine-123 conjugation The apoptosis assay showed increased apoptosis of cancer cells when treated with 5-FU compared to the normal cells These results indicated that novel 5-FU-TRC-NPs could be a promising candidate for cancer drug delivery (C) 2010 Elsevier Ltd All rights reserved
引用
收藏
页码:776 / 786
页数:11
相关论文
共 49 条
[31]   Nanoaggregate of thermosensitive chitosan-pluronic for sustained release of hydrophobic drug [J].
Park, Kyung Min ;
Bae, Jin Woo ;
Joung, Yoon Ki ;
Shin, Jung Woog ;
Park, Ki Dong .
COLLOIDS AND SURFACES B-BIOINTERFACES, 2008, 63 (01) :1-6
[32]  
PARTRIDGE AH, 2001, J NATL CANC I MONOGR, P135
[33]  
Peng SF, 2000, MACROMOL SYMP, V159, P179, DOI 10.1002/1521-3900(200010)159:1<179::AID-MASY179>3.0.CO
[34]  
2-8
[35]  
PEPPAS NA, 1985, PHARM ACTA HELV, V60, P110
[36]   Novel biodegradable chitosan-gelatin/nano-bioactive glass ceramic composite scaffolds for alveolar bone tissue engineering [J].
Peter, Mathew ;
Binulal, N. S. ;
Nair, S. V. ;
Selvamurugan, N. ;
Tamura, H. ;
Jayakumar, R. .
CHEMICAL ENGINEERING JOURNAL, 2010, 158 (02) :353-361
[37]   Development of novel α-chitin/nanobioactive glass ceramic composite scaffolds for tissue engineering applications [J].
Peter, Mathew ;
Kumar, Pandian Thodi Sudheesh ;
Binulal, Nelson Sathy ;
Nair, Shanthi V. ;
Tamura, Hiroshi ;
Jayakumar, Rangasamy .
CARBOHYDRATE POLYMERS, 2009, 78 (04) :926-931
[38]   Stimuli-responsive chitosan-graft-poly(N-vinylcaprolactam) as a promising material for controlled hydrophobic drug delivery [J].
Prabaharan, Mani ;
Grailer, Jamison J. ;
Steeber, Douglas A. ;
Gong, Shaoqin .
MACROMOLECULAR BIOSCIENCE, 2008, 8 (09) :843-851
[39]  
Radhakumary C, 2005, TRENDS BIOMATERIALS, V30, P117
[40]   Development of novel fibrinogen nanoparticles by two-step co-acervation method [J].
Rejinold, N. Sanoj ;
Muthunarayanan, M. ;
Deepa, N. ;
Chennazhi, K. P. ;
Nair, S. V. ;
Jayakumar, R. .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2010, 47 (01) :37-43