Dopamine depletion alters phosphorylation of striatal proteins in a model of Parkinsonism

被引:71
作者
Brown, AM
Deutch, AY
Colbran, RJ
机构
[1] Vanderbilt Univ, Sch Med, Dept Mol Physiol & Biophys, Nashville, TN 37232 USA
[2] Vanderbilt Univ, Sch Med, Dept Psychiat, Nashville, TN 37232 USA
[3] Vanderbilt Univ, Sch Med, Dept Pharmacol, Nashville, TN 37232 USA
[4] Vanderbilt Univ, Sch Med, Ctr Mol Neurosci, Nashville, TN 37232 USA
[5] Vanderbilt Univ, Sch Med, Ctr Integrat & Cognit Neurosci, Nashville, TN 37232 USA
[6] Vanderbilt Univ, Sch Med, Vanderbilt Kennedy Ctr Res Human Dev, Nashville, TN 37232 USA
关键词
ageing; AMPA receptor; CaMKII; DARPP-32; dendrite; postsynaptic density; PP1;
D O I
10.1111/j.1460-9568.2005.04190.x
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Nigrostriatal dopamine depletion disrupts striatal medium spiny neuron morphology in Parkinson's disease and modulates striatal synaptic plasticity in animal models of parkinsonism. We demonstrate that long-term nigrostriatal dopamine depletion in the rat induces evolving changes in the phosphorylation of striatal proteins critical for synaptic plasticity. Dopamine depletion increased the phosphorylation of the alpha isoform of calcium-calmodulin-dependent protein kinase II (CaMKII alpha) at Thr(286), a site associated with enhanced autonomous kinase activity, but did not alter total levels of CaMKII alpha or other synaptic proteins. Dopamine depletion decreased CaMKII alpha levels in postsynaptic density-enriched fractions without significant changes in other proteins. The activity of protein phosphatase 1 (PP1), a postsynaptic phosphatase that dephosphorylates CaMKII, is regulated by DARPP-32 (dopamine- and cAMP-regulated phosphoprotein of 32 kDa). Dopamine depletion had no effect on DARPP-32 phosphorylation at Thr(34), but increased DARPP-32 phosphorylation at Thr(75). Levodopa administration reversed the increased phosphorylation of both CaMKII alpha and DARPP-32. Normal ageing increased the levels of PP1(gamma 1 isoform) but decreased levels of the PP1 gamma 1-targeting proteins spinophilin and neurabin. Elevated phosphorylations of CaMKII alpha and DARPP-32 were maintained for up to 20 months after dopamine depletion. However, phosphorylation of the CaMKII-PP1 substrate, Ser(831) in the glutamate receptor GluR1 subunit, was increased only after sustained (9-20 months) dopamine depletion. Interaction of ageing-related changes in PP1 with the dopamine depletion-induced changes in CaMKII alpha may account for enhanced GluR1 phosphorylation only after long-term dopamine depletion. These evolving changes may impact striatal synaptic plasticity, Parkinson's disease progression and the changing efficacy and side-effects associated with dopamine replacement therapy.
引用
收藏
页码:247 / 256
页数:10
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