Temperature-regulated activity of responsive polymer-protein conjugates prepared by grafting-from via RAFT polymerization

被引:350
作者
De, Priyadarsi [1 ]
Li, Ming [1 ]
Gondi, Sudershan R. [1 ]
Sumerlin, Brent S. [1 ]
机构
[1] So Methodist Univ, Dept Chem, Dallas, TX 75275 USA
关键词
D O I
10.1021/ja804495v
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A facile route to well-defined "smart" polymer-protein conjugates with tunable bioactivity is reported. Protein modification with a reversible addition-fragmentation chain transfer (RAFT) agent and subsequent room temperature polymerization in aqueous media led to conjugates of poly(N-isopropylacrylamide) and a model protein. Representing the first example of polymer-protein conjugation with RAFT agent immobilization via the "R-group" approach, high molecular weight and reductively stable conjugates were accessible without extensive purification or adverse effects on the protein structure. An increase in molecular weight with conversion was observed for the chains grafted from the protein surface, confirming the controlled nature of the polymerization. The responsive behavior of the immobilized polymer facilitated conjugate isolation and also allowed environmental modulation of bioactivity.
引用
收藏
页码:11288 / +
页数:3
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