Synthesis, Characterization and Antibacterial Activity against Gram Positive and Gram Negative Bacteria of Biomimetically Coated Silver Nanoparticles

被引：171

作者：

Amato, Elvio ^{[1
]}

Diaz-Fernandez, Yuri A. ^{[1
]}

Taglietti, Angelo ^{[1
]}

Pallavicini, Piersandro ^{[1
]}

Pasotti, Luca ^{[1
]}

Cucca, Lucia ^{[1
]}

Milanese, Chiara ^{[2
,3
]}

Grisoli, Pietro ^{[4
]}

Dacarro, Cesare ^{[4
]}

Fernandez-Hechavarria, Jose M. ^{[5
]}

Necchi, Vittorio ^{[6
]}

机构：

[1] Univ Pavia, Dipartimento Chim, Sez Chim Gen, I-27100 Pavia, Italy

[2] Univ Pavia, CSGI, I-27100 Pavia, Italy

[3] Univ Pavia, Dipartimento Chim, Sez Chim Fis, I-27100 Pavia, Italy

[4] Univ Pavia, Dipartimento Sci Farm, I-27100 Pavia, Italy

[5] Inst Super Tecnol & Ciencias Aplicadas, Havana, Cuba

[6] Univ Pavia, Ctr Grandi Strumenti, I-27100 Pavia, Italy

来源：

LANGMUIR | 2011年 / 27卷 / 15期

关键词：

ESCHERICHIA-COLI; STAPHYLOCOCCUS-AUREUS; GOLD; IONS; AG; MICROSCOPY; CHEMISTRY; MECHANISM; TOXICITY; CYSTEINE;

D O I：

10.1021/la201200r

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In the present work, we describe a simple procedure to produce biomimetically coated silver nanopartides (Ag NPs), based on the postfunctionalization and purification of colloidal silver stabilized by citrate. Two biological capping agents have been used (cysteine Cys and glutathione GSH). The composition of the capped colloids has been ascertained by different techniques and antibacterial tests on GSH-capped Ag NPs have been conducted under physiological conditions, obtaining values of Minimum Inhibitory Concentration (MIC) of 180 and 15 mu g/mL for Staphylococcus aureus and Escherichia coli, respectively. The antibacterial activity of these GSH capped NPs can be ascribed to the direct action of metallic silver NPs, rather than to the bulk release of Ag+.

引用

页码：9165 / 9173

页数：9

共 64 条

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[2]

[Anonymous], 1999, Methods for determining bactericidal activity of antimicrobial agents

[3]

approved guideline M26-A, V19

[4]

[Anonymous], ZH NEORG KHIM