An RNA G-quadruplex in the 3′ UTR of the proto-oncogene PIM1 represses translation

被引：72

作者：

Arora, Amit ^{[1
]}

Suess, Beatrix ^{[1
]}

机构：

[1] Goethe Univ Frankfurt, Inst Mol Biowissensch, Frankfurt, Germany

来源：

RNA BIOLOGY | 2011年 / 8卷 / 05期

关键词：

PIM1; proto-oncogene; quadruplex; RNA; translation; HUMAN TELOMERE; K+ SOLUTION; 5'-UTR; 5-UTR; MOTIF;

D O I：

10.4161/rna.8.5.16038

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have identified a conserved G-quadruplex forming sequence in the 3' untranslated region (UTR) of the protooncogene PIM1. Circular dichroism and thermal denaturation studies revealed that the PIM1 mRNA guanine-rich sequence forms a highly stable intramolecular G-quadruplex structure. Reporter gene assay demonstrated that the PIM1 RNA G-quadruplex represses translation. It is the first experimental evidence of an RNA G-quadruplex structure located in the 3' UTR that may act as posttranscriptional regulator.

引用

页码：802 / 805

页数：4

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