Cytotoxicity of MTA and Portland cement on human ECV 304 endothelial cells

被引:66
作者
De Deus, G
Ximenes, R
Gurgel, ED
Plotkowski, MC
Coutinho, T
机构
[1] UERJ, Dept Endodont, Rio De Janeiro, Brazil
[2] UNIFOR, Dept Endodont, Fortaleza, Ceara, Brazil
[3] UERJ, Dept Microbiol, Rio De Janeiro, Brazil
关键词
cytotoxicity; mineral trioxide aggregate; portland cement;
D O I
10.1111/j.1365-2591.2005.00987.x
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Aim To evaluate the cytotoxic effects of two brands of mineral trioxide aggregate (MTA) (Pro-Root MTA((R)) and MTA Angelus((R))) and Portland cement (PC) on the human ECV 304 endothelial cell line. Methodology Endothelial ECV 304 cells were incubated at 37 degrees C in an atmosphere of 95% air, 5% carbon dioxide and 100% humidity for 7 days and grown in F12 medium supplemented with 10% fetal bovine serum with 50 mu g mL(-1) of gentamicin sulphate. Effects of the materials on mitochondrial functions were measured by a colorimetric assay. At each experimental time interval (24, 48 and 72 h), a dimethyl-thiazol-diphenyl tetrazolium bromid assay was conducted to measure cell viability. All assays were repeated three times to ensure reproducibility. Results were expressed as average absorbance (A(570 backslash,nm)) +/- SD and the data were analysed statistically by one-way analysis of variance and the Bonferroni post-test. A P-value < 0.05 was considered statistically significant. Results No statistically significant difference was shown between any of the experimental materials (P > 0.05). Conclusions The two brands of MTA analysed, as well as the PC, initially showed a similar elevated cytotoxic effect that decreased gradually with time allowing the cell culture to become reestablished.
引用
收藏
页码:604 / 609
页数:6
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