Phycobiliproteins from Arthrospira Platensis (Spirulina): A New Source of Peptides with Dipeptidyl Peptidase-IV Inhibitory Activity

被引：73

作者：

Li, Yuchen ^{[1
]}

Aiello, Gilda ^{[1
]}

Bollati, Carlotta ^{[1
]}

Bartolomei, Martina ^{[1
]}

Arnoldi, Anna ^{[1
]}

Lammi, Carmen ^{[1
]}

机构：

[1] Univ Milan, Dept Pharmaceut Sci, I-20133 Milan, Italy

来源：

NUTRIENTS | 2020年 / 12卷 / 03期

关键词：

allophycocyanin; bioactive peptides; C-Phycocyanin; DPP-IV; LC-MS; MS; Spirulina platensis; C-PHYCOCYANIN; PATHWAY;

D O I：

10.3390/nu12030794

中图分类号：

R15 [营养卫生、食品卫生]; TS201 [基础科学];

学科分类号：

100403 [营养与食品卫生学];

摘要：

Arthrospira platensis (spirulina) is a cyanobacterium, which contains mainly two phycobiliproteins (PBP), i.e., C-phycocyanin (C-PC) and allophycocyanin (APC). In this study, PBP were hydrolyzed using trypsin, and the composition of the hydrolysate was characterized by HPLC-ESI-MS/MS. Furthermore, the potential anti-diabetic activity was assessed by using either biochemical or cellular techniques. Findings suggest that PBP peptides inhibit DPP-IV activity in vitro with a dose-response trend and an IC50 value falling in the range between 0.5 and 1.0 mg/mL. A lower inhibition of the DPP-IV activity expressed by Caco-2 cells was observed, which was explained by a secondary metabolic degradation exerted by the same cells.

引用

页数：11

共 30 条

[1]

Chemical and biological characterization of spirulina protein hydrolysates: Focus on ACE and DPP-IV activities modulation [J].