Structure, expression profile and chromosomal location of an isolog of DNA-PKcs interacting protein (KIP) gene

被引:23
作者
Seki, N
Hattori, A
Hayashi, A
Kozuma, S
Ohira, M
Hori, T
Saito, T
机构
[1] Natl Inst Radiol Sci, Genome Res Grp, Inage Ku, Chiba 2638555, Japan
[2] Kazusa DNA Res Inst, Lab Gene Funct 2, Chiba 2920812, Japan
[3] Aisin Cosmos R&D Co Ltd, Biotechnol & Med Engn Field, Chiyoda Ku, Tokyo 1010021, Japan
[4] Chiba Canc Ctr, Div Biochem, Chuoh Ku, Chiba 2608717, Japan
来源
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1999年 / 1444卷 / 01期
关键词
D O I
10.1016/S0167-4781(98)00253-X
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A novel DNA-PKcs interacting protein, KIP (kinase interacting protein), was recently isolated using a two-hybrid analysis which showed a significant homology to calcineurin B. We found other ESTs showing significant similarity to KIP gene in the dbEST database and isolated a cDNA clone which encodes a 187 amino acid polypeptide from a human fetal brain cDNA library. This protein (termed KIP2 for kinase interacting protein 2) has sequence homology to KIP (46% identical and 64% similarity). RT-PCR analysis showed that the messenger RNA was ubiquitously expressed in various human tissues. Based on PCR-based analysis with a radiation hybrid cell panel and fluorescence in situ hybridization, the gene was localized to the q24 region of chromosome 15. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:143 / 147
页数:5
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