Reconstitution of the blood-brain barrier in cell culture for studies of drug transport and metabolism

被引：13

作者：

deBoer, AG

Breimer, DD

机构：

[1] Division of Pharmacology, Leiden/Amsterdam Ctr. for Drug Res., University of Leiden, 2300 RA Leiden

来源：

ADVANCED DRUG DELIVERY REVIEWS | 1996年 / 22卷 / 1-2期

关键词：

anatomy; in vitro; isolation; visualization; inflammation;

D O I：

10.1016/S0169-409X(96)00421-8

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

In this paper the anatomy of the blood-brain barrier and the properties of peripheral versus brain-microvessel-endothelial cells (BMEC) are outlined and the isolation and application of microvessels are discussed. In addition, the isolation and culture of BMEC are described and discussed and the application of this system in drug transport- and metabolism-studies, visualization of drug transport routes, and in the study of changes in BBB functionality during inflammation, is illustrated.

引用

收藏

页码：251 / 264

页数：14

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