共 43 条
TrkA receptor endolysosomal degradation is both ubiquitin and proteasome dependent
被引:48
作者:

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Wooten, Marie W.
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Auburn Univ, Dept Biol Sci, Program Cellular & Mol Biosci, Auburn, AL 36849 USA Auburn Univ, Dept Biol Sci, Program Cellular & Mol Biosci, Auburn, AL 36849 USA
机构:
[1] Auburn Univ, Dept Biol Sci, Program Cellular & Mol Biosci, Auburn, AL 36849 USA
来源:
关键词:
degradation;
DUBs;
internalization;
lysosomes;
proteasome;
TrkA;
ubiquitin;
D O I:
10.1111/j.1600-0854.2008.00751.x
中图分类号:
Q2 [细胞生物学];
学科分类号:
071009 ;
090102 ;
摘要:
Gaps in our knowledge exist regarding the degradation of the tropomyosin-regulated kinase A (TrkA) receptor after addition of neurotrophin, nerve growth factor (NGF). TrkA is rapidly and transiently ubiquitinated upon addition of NGF. Here, we demonstrate that the polyubiquitin tag plays a definitive role in receptor sorting. Treatment of PC12 cells with lactacystin prevented NGF-dependent deubiquitination and degradation of TrkA. However, treatment with methylamine, bafilomycin or leupeptin, did not prevent NGF-dependent deubiquitination but blocked the degradation of TrkA. Employing co-immunoprecipitation, biochemical fractionation and confocal microscopy, the kinetics of receptor trafficking post-internalization was observed to occur as a sequel from endosome/multivesicular body, proteasomes, culminating with degradation in the lysosomes. The trafficking of the polyubiquitin-deficient TrkA receptor mutant K485R was impaired and likewise failed to degrade revealing that the receptor escapes degradation. The interaction of TrkA with proteasomes was confirmed by purification and co-immunoprecipitation. We provide evidence that proteasomal deubiquitinating enzymes trim K63-ubiquitin chains from the TrkA receptor prior to its delivery to lysosomes for degradation. Taken together, our results reveal the existence of a novel proteasome-dependent step in receptor degradation.
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页码:1146 / 1156
页数:11
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