Progress in analytical imaging of the cell by dynamic secondary ion mass spectrometry (SIMS microscopy)

被引:197
作者
Guerquin-Kern, JL [1 ]
Wu, TD [1 ]
Quintana, C [1 ]
Croisy, A [1 ]
机构
[1] Ctr Univ Orsay, Lab Microscopie Inon, Inst Curie Rech, F-91405 Orsay, France
来源
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 2005年 / 1724卷 / 03期
基金
澳大利亚研究理事会;
关键词
ultrastructure; cell imaging; ion microscopy; dynamic secondary ion mass spectrometry; parallel detection; stable isotope;
D O I
10.1016/j.bbagen.2005.05.013
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
This paper reviews the most recent methodological advances in the field of biological imaging using dynamic secondary ion mass spectrometry (SIMS). After a short reminder of the basic principle of SIMS imaging, the latest high-resolution dynamic SIMS equipment is briefly described. This new ion nanoprobe (CAMECA NanoSIMS 50 (TM)) has a lateral resolution of less than 50 nm with primary Cs+ ion, the ability to detect simultaneously 5 different ions from the same micro-volume and a very good transmission even at high mass resolution (60% at M/Delta M=5000). Basic considerations related to sample preparation, mass resolution and primary ion implantation are given. The decisive capability of this new instrument, and more generally of high-resolution dynamic SIMS imaging in biology, are illustrated with the most recent examples of utilization(2). (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:228 / 238
页数:11
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