Selection of Lactobacillus plantarum TN627 as a new probiotic candidate based on in vitro functional properties

被引：9

作者：

Bejar, Wacim ^{[1
]}

Farhat-Khemakhem, Ameny ^{[1
]}

Smaoui, Slim ^{[1
]}

Makni, Mohamed ^{[2
]}

Ben Farhat, Mounira ^{[1
]}

Abdelmalek, Badis ^{[3
]}

Mellouli, Lotfi ^{[1
]}

Maguin, Emmanuelle ^{[4
]}

Bejar, Samir ^{[1
]}

Chouayekh, Hichem ^{[1
]}

机构：

[1] Univ Sfax, LMB, CBS, Sfax 3018, Tunisia

[2] Fac Sci Sfax, Physiol Anim Lab, Sfax 3000, Tunisia

[3] Univ Saad Dahlab Blida, Pedag Biochim & Microbiol Ind LPBMI, Dept Chim Ind, Blida 09000, Algeria

[4] INRA, Unite Genet Microbienne, UR895, F-78352 Jouy En Josas, France

来源：

BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | 2011年 / 16卷 / 06期

关键词：

Lactobacillus plantarum TN627; probiotic potential; antibacterial activity; sensitivity to antibiotics; adhesion capacity; LACTIC-ACID BACTERIA; GROWTH PROMOTERS; IDENTIFICATION; TYPHIMURIUM; ADHESION; SEQUENCE;

D O I：

10.1007/s12257-011-0198-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

Nine lactobacilli previously selected for high antagonism against food borne bacterial pathogens were identified via 16S rRNA gene sequencing and screened for probiotic potential for use in poultry production. The lactobacilli were subjected to a subtractive in vitro analysis system using a certified probiotic as reference. This allowed for selection of a milk-derived Lactobacillus plantarum strain, termed TN627. This organic acid-producing bacterium was free of harmful enzymatic activity and sensitive to several antibiotics. It also showed good growth at pH 4 and in the presence of bile. L. plantarum TN627 also exhibited high efficacy of adhesion to chicken enterocytes, which correlated with detecting genes encoding the mucusbinding, adhesion-promoting proteins (Mub and MapA) and the adhesion-like factor EF-Tu, commonly involved in adherence of lactobacilli to mucosal surfaces. Taken together, our findings suggest that TN627 is a promising probiotic candidate with high potential for application as a supplement in the animal feed industry.

引用

页码：1115 / 1123

页数：9

共 34 条

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