Development and optimization of a method for analyzing biodiesel mixtures with non-aqueous reversed phase liquid chromatography

被引:42
作者
Di Nicola, G. [1 ]
Pacetti, M. [1 ]
Polonara, F. [1 ]
Santori, G. [1 ]
Stryjek, R. [1 ,2 ]
机构
[1] Univ Politecn Marche, Fac Ingn, Dipartimento Energet, I-60131 Ancona, Italy
[2] Polish Acad Sci, Inst Phys Chem, Warsaw, Poland
关键词
biodiesel; HPLC; sunflower oil; factorial design; optimization; transesterification;
D O I
10.1016/j.chroma.2008.02.085
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Biodiesel (a mixture of fatty acid esters) is normally analyzed using gas chromatography/flame ionization detection, as specified by the ASTM D6584 and EN14105 standards. This paper proposes a binary gradient method for analyzing biodiesel mixtures using non-aqueous reverse phase HPLC with a UV detector capable of overcoming the drawbacks of the gas chromatographic technique normally used. The new analytical method was developed by means of a statistical sensitivity analysis applied to the main parameters influencing the recording, using the full factorial design method combined with the Yates algorithm and the steepest ascent optimization procedure. The present study shows the influence of the main biodiesel mixture separation analysis parameters. The resulting tool proved valid for analyzing not only biodiesel but also any traces of unreacted oil. (C) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码:120 / 126
页数:7
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