CueO is a multi-copper oxidase that confers copper tolerance in Escherichia coli

被引：250

作者：

Grass, G ^{[1
]}

Rensing, C ^{[1
]}

机构：

[1] Univ Arizona, Dept Soil Water & Environm Sci, Tucson, AZ 85721 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2001年 / 286卷 / 05期

关键词：

copper; Escherichia coli; multi-copper oxidase; metal homeostasis; laccase; periplasm;

D O I：

10.1006/bbrc.2001.5474

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The putative multi-copper oxidase CueO had previously been implicated in intrinsic copper resistance in Escherichia coli. In this report we showed that the presence of CueO in the periplasm protected alkaline phosphatase from copper-induced damage. CueO contained four. copper atoms per molecule and displayed spectroscopic properties typical of blue copper oxidases. CueO catalyzed the oxidation of p-phenylene-diamine (pPD), 2,6-dimethoxyphenol (DMP) and exhibited ferroxidase activity in vitro. (C) 2001 Academic Press.

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页码：902 / 908

页数：7

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