Heme oxygenase-1 (Hsp32) is involved in the protection of small intestine by whole body mild hyperthermia from ischemia/reperfusion injury in rat

被引：48

作者：

Sakamoto, N ^{[1
]}

Kokura, S ^{[1
]}

Okuda, T ^{[1
]}

Hattori, T ^{[1
]}

Katada, K ^{[1
]}

Isozaki, Y ^{[1
]}

Nakabe, N ^{[1
]}

Handa, O ^{[1
]}

Takagi, T ^{[1
]}

Ishikawa, T ^{[1
]}

Naito, Y ^{[1
]}

Yoshida, N ^{[1
]}

Yoshikawa, T ^{[1
]}

机构：

[1] Kyoto Prefectural Univ Med, Dept Biomed Safety Sci, Kamigyo Ku, Kyoto 6028566, Japan

来源：

INTERNATIONAL JOURNAL OF HYPERTHERMIA | 2005年 / 21卷 / 07期

关键词：

ischemia-reperfusion; heat shock protein; heme oxygenase-1; hyperthermia; Hsp70;

D O I：

10.1080/02656730500188599

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

Aim: The aim of the present study was to explore whether heme oxygenase-1 (HO-1) is involved in the hyperthermia-provided protection of the small intestine from ischemia/reperfusion injury in rats. Methods: Intestinal damage was induced in male Sprague-Dawley rats by clamping both the superior mesenteric artery and the celiac trunk for 30 min, followed by reperfusion. Whole-body hyperthermia was induced in anesthetized rats by placement in a temperature-controlled water bath. Whole-body hyperthermia to a core temperature of 42-43 degrees C for 15 min was followed by passive cooling. We started the hyperthermic treatment 6 h before the vascular clamping. The severity of the mucosal injury was evaluated by several biochemical markers and histological findings. Hyperthermia-induced heat-shock proteins were detected by Western blotting. We also investigated the effect of zinc protoporphyrin IX (an HO-1 inhibitor) on the protective effect of hyperthermia. Results: The rats, which were killed after ischemia/reperfusion, had severe intestinal inflammation. Hyperthermia significantly induced the production of Hsp70 and HO-1 in intestinal mucosa and significantly reduced ischemia/reperfusion-induced mucosal injury. The combination of zinc protoporphyrin IX with hyperthermia extinguished the protective effects of hyperthermia on ischemia/reperfusion injury. Conclusion: Hyperthermia protects against ischemia/reperfusion injury in rat small intestine through the expression of heat-shock proteins, especially HO-1.

引用

页码：603 / 614

页数：12

共 25 条

[1]

Heat shock treatment suppresses angiotensin II-induced activation of NF-κB pathway and heart inflammation:: a role for IKK depletion by heat shock? [J].