pH sensing in living cells using fluorescent microspheres

被引：80

作者：

Bradley, Mark ^{[1
]}

Alexander, Lois ^{[1
]}

Duncan, Karen ^{[1
]}

Chennaoui, Mourad ^{[1
]}

Jones, Anita C. ^{[2
]}

Sanchez-Martin, Rosario M. ^{[1
]}

机构：

[1] Univ Edinburgh, Sch Chem, Chem Biol Sect, Edinburgh EH9 3JJ, Midlothian, Scotland

[2] Univ Edinburgh, Sch Chem, Chem Phys Sect, Edinburgh EH9 3JJ, Midlothian, Scotland

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2008年 / 18卷 / 01期

基金：

英国医学研究理事会; 英国工程与自然科学研究理事会;

关键词：

D O I：

10.1016/j.bmcl.2007.10.075

中图分类号：

R914 [药物化学];

学科分类号：

100701 [药物化学];

摘要：

Intracellular pH in living cells is measured in real time at the single cell level using fluorescently covalently loaded microspheres as efficient carrier systems and stable sensors. The use of these sensors immobilized covalently onto polymeric particles allows analysis of intracellular pH flux over long period of time and eliminates the disadvantages such as dilution within the cell, elimination via leakage or compartmentalization. (C) 2007 Elsevier Ltd. All rights reserved.

引用

页码：313 / 317

页数：5

共 20 条

[1]

Development of a novel GFP-based ratiometric excitation and emission pH indicator for intracellular studies [J].