Poly(dG)-poly(dC) DNA appears shorter than poly(dA)-poly(dT) and possibly adopts an A-related conformation on a mica surface under ambient conditions

被引：11

作者：

Borovok, Natalia ^{[2
]}

Molotsky, Tatiana ^{[2
]}

Ghabboun, Jamal ^{[1
]}

Cohen, Hezy ^{[1
]}

Porath, Danny ^{[1
,3
]}

Kotlyar, Alexander ^{[2
,4
]}

机构：

[1] Hebrew Univ Jerusalem, Dept Phys Chem, IL-91904 Jerusalem, Israel

[2] Tel Aviv Univ, George S Wise Fac Life Sci, Dept Biochem, IL-69978 Tel Aviv, Israel

[3] Hebrew Univ Jerusalem, Ctr Nanosci & Nanotechnol, IL-91904 Jerusalem, Israel

[4] Tel Aviv Univ, Nantechnol Ctr, IL-69978 Tel Aviv, Israel

来源：

FEBS LETTERS | 2007年 / 581卷 / 30期

关键词：

atomic force microscopy (AFM); DNA imaging; Poly(dG)-poly(dC); Poly(dA)-poly(dT); A-form;

D O I：

10.1016/j.febslet.2007.11.058

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Three types of DNA: similar to 2700 bp polydeoxyguanylic olydeoxycytidylic acid [poly(dG)-poly(dC)], similar to 2700 bp polydeoxyadenylic polydeoxythymidylic acid [poly(dA)-poly(dT)] and 2686 bp linear plasmid pUC19 were deposited on a mica surface and imaged by atomic force microscopy. Contour length measurements show that the average length of poly(dG)-poly(dC) is similar to 30% shorter than that of poly(dA)-poly(dT) and the plasmid. This led us to suggest that individual poly(dG)-poly(dC) molecules are immobilized on mica under ambient conditions in a form which is likely related to the A-form of DNA in contrast to poly(dA)-poly(dT) and random sequence DNA which are immobilized in a form that is related to the DNA B-form. (C) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：5843 / 5846

页数：4

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