Hsp70 duplication in the Drosophila melanogaster species group:: How and when did two become five?

被引:62
作者
Bettencourt, BR
Feder, ME
机构
[1] Univ Chicago, Dept Organismal Biol & Anat, Chicago, IL 60637 USA
[2] Univ Chicago, Comm Evolutionary Biol, Chicago, IL 60637 USA
关键词
Drosophila; hsp70; gene duplication; concerted evolution;
D O I
10.1093/oxfordjournals.molbev.a003912
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
To determine how the modem copy number (5) of hsp70 genes in Drosophila melanogaster evolved, we localized the duplication events that created the genes in the phylogeny of the melanogaster group, examined D. melanogaster genomic sequence to investigate the mechanisms of duplication, and analyzed the hsp70 gene sequences of Drosophila orena and Drosophila mauritiana. The initial two-to-four hsp70 duplication occurred 10-15 MYA, according to fixed in situ hybridization to polytene chromosomes, before the origin and divergence of the melanogaster and five: other species subgroups of the, melanogaster group. Analysis of more than 30 kb of flanking sequence surrounding the hsp70 gene clusters suggested that this duplication was Likely a retrotransposition. For the melanogaster subgroup, Southern hybridization and an hsp70 restriction map confirmed the conserved number (4) and arrangement of hsp70 genes in the seven species other than D. melanogaster. Drosophila melanogaster is unique; tandem duplication and gene conversion at the derived cluster yielded a fifth hsp70 gene. The four D. orena hsp70 genes are highly similar and concertedly evolving. In contrast, the D. mauritiana hsp70 genes are divergent, and many alleles. are nonfunctional. The proliferation, concerted evolution, and maintenance of functionality in the D. melanogaster hsp70 genes is consistent with the, action of natural selection in this species.
引用
收藏
页码:1272 / 1282
页数:11
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