The art and design of genetic screens: RNA interference

被引：308

作者：

Boutros, Michael ^{[1
,4
]}

Ahringer, Julie ^{[2
,3
]}

机构：

[1] Heidelberg Univ, Div Signalling & Funct Genom, German Canc Res Ctr, D-69120 Heidelberg, Germany

[2] Univ Cambridge, Dept Genet, Cambridge CB2 1QN, England

[3] Univ Cambridge, Gurdon Inst, Cambridge CB2 1QN, England

[4] Heidelberg Univ, CBTM, D-69120 Heidelberg, Germany

来源：

NATURE REVIEWS GENETICS | 2008年 / 9卷 / 07期

基金：

英国惠康基金;

关键词：

D O I：

10.1038/nrg2364

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The remarkable gene knockdown technique of RNAi has opened exciting new avenues for genetic screens in model organisms and human cells. Here we describe the current state of the art for RNAi screening, and stress the importance of well-designed assays and of analytical approaches for large-scale screening experiments, from high-throughput screens using simplified homogenous assays to microscopy and whole-animal experiments. Like classical genetic screens in the past, the success of large-scale RNAi surveys depends on a careful development of phenotypic assays and their interpretation in a relevant biological context.

引用

收藏

页码：554 / 566

页数：13

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