Subdiffraction-resolution fluorescence imaging with conventional fluorescent probes

被引：1341

作者：

Heilemann, Mike ^{[1
]}

van de Linde, Sebastian ^{[1
]}

Schuttpelz, Mark ^{[1
]}

Kasper, Robert ^{[1
]}

Seefeldt, Britta ^{[1
]}

Mukherjee, Anindita ^{[1
]}

Tinnefeld, Philip ^{[2
]}

Sauer, Markus ^{[1
]}

机构：

[1] Univ Bielefeld, D-33615 Bielefeld, Germany

[2] Univ Munich, Ctr NanoSci, D-80799 Munich, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2008年 / 47卷 / 33期

关键词：

fluorescent probes; imaging agents; localization microscopy; optical switches; subdiffraction resolution;

D O I：

10.1002/anie.200802376

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Graph Presented) Eagle eyes: dSTORM uses conventional photoswitchable fluorescent dyes that can be reversibly cycled between a fluorescent and a dark state by irradiation with light of different wavelengths (see picture). This elegant approach can visualize cellular structures with a resolution of approximately 20 nm, far beyond the diffraction limit of light, without the need of an activator molecule. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

收藏

页码：6172 / 6176

页数：5

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