The relationship of chronic mucin secretion to airway disease in normal and CFTR-deficient mice

被引:39
作者
Cressman, VL
Hicks, EM
Funkhouser, WK
Backlund, DC
Koller, BH
机构
[1] Univ N Carolina, Curriculum Genet & Mol Biol, Dept Med, Chapel Hill, NC 27599 USA
[2] Univ N Carolina, Dept Pathol & Lab Med, Chapel Hill, NC 27599 USA
关键词
D O I
10.1165/ajrcmb.19.6.3194
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In the cystic fibrosis (CF) patient, lung function decreases throughout life as a result of continuous cycles of infection, particularly with Pseudomonas aeruginosa and Staphylococcus aureus. The mechanism underlying the pathophysiology of the disease in humans has not been established. However, it has been suggested that abnormal, tenacious mucus, resulting perhaps from improper hydration from loss of Cl- secretion via the cystic fibrosis transmembrane conductance regulator (CFTR) protein, impairs clearance of bacteria from the CF airway and provides an environment favorable to bacterial growth. If this hypothesis is correct, it could explain the absence of respiratory disease in CFTR-deficient mice, since mice have only a single submucosal gland and display few goblet cells in their lower airways, even when exposed to bacteria. To test this hypothesis further, we induced allergic airway disease in CFTR-deficient mice. We found that induction of allergic airway disease in mice, unlike bacterial infection, results in an inflammatory response characterized by goblet cell hyperplasia, increased mucin gene expression, and increased production of mucus. However, we also found that disease progression and resolution is identical in Cftr(-/-) mice and control animals. Furthermore, we show that the presence of mucus in the Cftr(-/-) airway does not lead to chronic airway disease, even upon direct inoculation with S. aureus and P. aeruginosa. Therefore, factors in addition to the absence of high levels of mucus secretion protect the mouse from the airway disease seen in human CF patients.
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页码:853 / 866
页数:14
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