Scooby-domain: prediction of globular domains in protein sequence

被引：25

作者：

George, RA

Lin, K

Heringa, J

机构：

[1] Vrije Univ Amsterdam, Ctr Integrat Bioinformat, IBIVU, Fac Sci, NL-1081 HV Amsterdam, Netherlands

[2] Vrije Univ Amsterdam, Fac Earth & Life Sci, NL-1081 HV Amsterdam, Netherlands

[3] Inparmat Ltd, London W1T 2NU, England

[4] European Bioinformat Inst, Cambridge CB10 1SD, England

[5] Natl Inst Med Res, Div Math Biol, London NW7 1AA, England

来源：

NUCLEIC ACIDS RESEARCH | 2005年 / 33卷

基金：

英国惠康基金;

关键词：

D O I：

10.1093/nar/gki381

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Scooby-domain (sequence hydrophobicity predicts domains) is a fast and simple method to identify globular domains in protein sequence, based on the observed lengths and hydrophobicities of domains from proteins with known tertiary structure. The prediction method successfully identifies sequence regions that will form a globular structure and those that are likely to be unstructured. The method does not rely on homology searches and, therefore, can identify previously unknown domains for structural elucidation. Scooby-domain is available as a Java applet at http:// ibivu.cs.vu.nl/programs/scoobywww. It may be used to visualize local properties within a protein sequence, such as average hydrophobicity, secondary structure propensity and domain boundaries, as well as being a method for fast domain assignment of large sequence sets.

引用

页码：W160 / W163

页数：4

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