Genes involved in cadmium resistance in Caulobacter crescentus

被引：21

作者：

Braz, VS ^{[1
]}

Marques, MV ^{[1
]}

机构：

[1] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, BR-05508900 Sao Paulo, Brazil

来源：

FEMS MICROBIOLOGY LETTERS | 2005年 / 251卷 / 02期

基金：

巴西圣保罗研究基金会;

关键词：

cadmium resistance; resistance-nodulation-cell division family; Caulobacter;

D O I：

10.1016/j.femsle.2005.08.013

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A transposon library of 5700 mutants was constructed in Caulobacter crescentus strain NA1000. Thirteen mutant strains were isolated that presented growth deficiency in the presence of 20 mu M cadmium chloride, and the disrupted genes were identified by DNA sequencing. Among the predicted products were found two putative transcriptional regulators, one histidine kinase/response regulator hybrid protein, two 2Fe-2S family proteins, and also members of a putative metal efflux system. Certain of these mutants also showed an increased sensitivity to zinc and copper, and the genes encoding the efflux system were induced by cadmium and zinc, indicating that this system may be important for homeostasis of both metal ions. (C) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：289 / 295

页数：7

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