Expression of the mammalian homologue of vacuolar protein sorting 16 (Vps16p) in the mouse and rat brain

被引：7

作者：

Kim, BY ^{[1
]}

Ueda, M ^{[1
]}

Nakamura, Y ^{[1
]}

Kohsaka, S ^{[1
]}

Akazawa, C ^{[1
]}

机构：

[1] Natl Inst Neurosci, Dept Neurochem, NCNP, Kodaira, Tokyo 1878502, Japan

来源：

NEUROSCIENCE LETTERS | 2004年 / 355卷 / 03期

关键词：

membrane fusion; synaptic transmission; membrane traffic; soluble N-ethylmaleimide-sensitive factor;

D O I：

10.1016/j.neulet.2003.11.021

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Synaptic vesicle fusion events are essential for synaptic transmission. Membrane docking and fusion events are highly regulated processes requiring the participation of a large number of soluble N-ethylmaleimide-sensitive factors (SNAREs) and SNARE-interacting proteins. We report the neuronal expression of mammalian homologue of vacuolar protein sorting 16 (mVps16p) which exhibits a high homology to the yeast Vps16p, a component of Class C Vps. Western blot and immunohistochemical analyzes revealed that mVps16p is highly expressed in the various brain areas and developmental stages tested. The immunoreactivities of mVps16p colocalized with microtubule associated protein 2, but not glial fibrillary acidic protein. In the primary culture of cortical neurons, mVps16p immunoreactivities were observed in the cell body and the neuronal processes, and highly enriched in the axonal outgrowths. (C) 2003 Elsevier Ireland Ltd. All rights reserved.

引用

页码：217 / 220

页数：4

共 13 条

[1] Thalamocortical synaptic connections: Efficacy, modulation, inhibition and plasticity [J].

Amitai, Y .

REVIEWS IN THE NEUROSCIENCES, 2001, 12 (02) :159-173

[2]

Braunewell KH, 2001, REV NEUROSCIENCE, V12, P121

[3] Staring, a novel E3 ubiquitin-protein ligase that targets syntaxin 1for degradation [J].

Chin, LS ;

Vavalle, JP ;

Li, L .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (38) :35071-35079

[4] Identification of mouse Vps16 and biochemical characterization of mammalian Class C Vps complex [J].

Kim, BY ;

Ueda, M ;

Kominami, E ;

Akagawa, K ;

Kohsaka, S ;

Akazawa, C .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2003, 311 (03) :577-582

[5] Molecular characterization of mammalian homologues of class CVPS proteins that interact with syntaxin-7 [J].

Kim, BY ;

Krämer, H ;

Yamamoto, A ;

Kominami, E ;

Kohsaka, S ;

Akazawa, C .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (31) :29393-29402

[6] DEVELOPMENTAL EXPRESSION OF THE 25-KDA SYNAPTOSOMAL-ASSOCIATED PROTEIN (SNAP-25) IN RAT-BRAIN [J].

OYLER, GA ;

POLLI, JW ;

WILSON, MC ;

BILLINGSLEY, ML .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (12) :5247-5251

[7] N-SEC1 - A NEURAL-SPECIFIC SYNTAXIN-BINDING PROTEIN [J].

PEVSNER, J ;

HSU, SC ;

SCHELLER, RH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (04) :1445-1449

[8] NEUROTRANSMISSION HARNESSING FUSION MACHINERY AT THE SYNAPSE [J].

SOLLNER, T ;

ROTHMAN, JE .

TRENDS IN NEUROSCIENCES, 1994, 17 (08) :344-348

[9] SNAP RECEPTORS IMPLICATED IN VESICLE TARGETING AND FUSION [J].

SOLLNER, T ;

WHITEHART, SW ;

BRUNNER, M ;

ERDJUMENTBROMAGE, H ;

GEROMANOS, S ;

TEMPST, P ;

ROTHMAN, JE .

NATURE, 1993, 362 (6418) :318-324

[10] SNAP-29: A general SNARE protein that inhibits SNARE disassembly and is implicated in synaptic transmission [J].

Su, QN ;

Mochida, S ;

Tian, JH ;

Mehta, R ;

Sheng, ZH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (24) :14038-14043

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