Defining the repeating elements in the cysteine-rich region (CRR) of the CD18 integrin β2 subunit

被引：14

作者：

Tan, SM

Walters, SE

Mathew, EC

Robinson, MK

Drbal, K

Shaw, JM

Law, SKA

机构：

[1] Univ Oxford, Dept Biochem, MRC, Immunochem Unit, Oxford, England

[2] Celltech Plc, Slough, Berks, England

[3] Acad Sci Czech Republ, Inst Mol Genet, Prague, Czech Republic

来源：

FEBS LETTERS | 2001年 / 505卷 / 01期

关键词：

integrin; beta subunit; cysteine-rich region;

D O I：

10.1016/S0014-5793(01)02778-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cysteine-rich region (CRR) of the integrin beta subunits is organised into four repeating elements. By expression of a panel of truncated beta2 subunits, and CRR segments fused to the C-terminal end of a CD4 soluble fragment, the segment required for the expression of two monoclonal antibody conformational epitopes was determined. This segment, E482-Q574, contains 16 cysteines representing two repeating units. We have thus defined the CRR unit motif of 'xC-C-C-CxCxxCxC-Cx', where 'x' represents a single residue, and represents a stretch of four to 14 residues. (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

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收藏

页码：27 / 30

页数：4

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