Myoglobin immunoassay based on metal particle-enhanced fluorescence

被引：70

作者：

Matveeva, EG ^{[1
]}

Gryczynski, Z ^{[1
]}

Lakowicz, JR ^{[1
]}

机构：

[1] Univ Maryland, Sch Med, Ctr Fluorescence Spectroscopy, Dept Biochem & Mol Biol, Baltimore, MD 21201 USA

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2005年 / 302卷 / 1-2期

关键词：

myoglobin; cardiac markers; fluorescence immunoassay; metal-enhanced fluorescence; nano-size metallic particles; silver island films;

D O I：

10.1016/j.jim.2005.04.020

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Enhanced fluorescence on silver island films (SIFs) is utilized to develop a sandwich-format immunoassay for the cardiac marker myoglobin (Myo). Myoglobin was first captured on surfaces coated with anti-Myo antibodies; the surface was then incubated with fluorescently labeled anti-Myo antibodies. The system was examined on glass surfaces and on SIFs. We observed the enhancement of the signal from SIFs in the range of 10-15-fold if compared to the signal from the glass substrate not modified with a SIR A kinetic immunoassay for Myo on SIF-modified surface results in a decreased background signal. The initial results show that it is possible to detect Myoglobin concentrations below 50 ng/mL, which is lower than clinical cut-off for Myoglobin in healthy patients. We suggest the use of SIF-modified substrates for increasing the sensitivity of surface assays with fluorescence detection. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：26 / 35

页数：10

共 33 条

[11]

Gibler W Brian, 2003, Rev Cardiovasc Med, V4 Suppl 4, pS47

[12] Synthesis and optical properties of anisotropic metal nanoparticles [J].