SFC/MS in drug discovery at Pfizer, La Jolla

被引：71

作者：

Bolaños, B ^{[1
]}

Greig, M ^{[1
]}

Ventura, M ^{[1
]}

Farrell, W ^{[1
]}

Aurigemma, CM ^{[1
]}

Li, H ^{[1
]}

Quenzer, TL ^{[1
]}

Tivel, K ^{[1
]}

Bylund, JMR ^{[1
]}

Tran, P ^{[1
]}

Pham, C ^{[1
]}

Phillipson, D ^{[1
]}

机构：

[1] Pfizer Global Res & Dev, San Diego, CA 92121 USA

来源：

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY | 2004年 / 238卷 / 02期

关键词：

supercritical fluid; mass spectrometry; peptide; photoionization;

D O I：

10.1016/j.ijms.2003.11.021

中图分类号：

O64 [物理化学（理论化学）、化学物理学]; O56 [分子物理学、原子物理学];

学科分类号：

070203 ; 070304 ; 081704 ; 1406 ;

摘要：

We report the use of supercritical fluid chromatography/mass spectrometry (SFC/MS) for numerous applications in drug discovery at Pfizer, La Jolla. Namely, SFC/MS has been heavily relied upon for analysis and purification of a diverse set of compounds from the in-house chemical library. Supporting high-speed SFC/MS quality control of the purified compounds is made possible at high flow rate SFC along with time-of-flight mass detection. The flexibility of SFC/MS systems has been extended with the integration of an atmospheric pressure photoionization source (APPI) for use with more non-polar compounds and enhancements in signal to noise. Further SFC/MS applications of note include chiral analysis for purification and assessment of enantiomers and SFC/MS analysis of difficult to separate hydrophobic peptides. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：85 / 97

页数：13

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