A gold nanoparticle-based aptamer target binding readout for ATP assay

被引：376

作者：

Wang, Jing

Wang, Lihua ^{[1
]}

Liu, Xingfen

Liang, Zhiqiang

Song, Shiping

Li, Wenxin

Li, Genxi

Fan, Chunhai

机构：

[1] Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China

[2] Nanjing Univ, Dept Biochem, Nanjing 210093, Peoples R China

[3] Nanjing Univ, Natl Key Lab Pharmaceut Biotechnol, Nanjing 210093, Peoples R China

[4] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

来源：

ADVANCED MATERIALS | 2007年 / 19卷 / 22期

关键词：

D O I：

10.1002/adma.200602256

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A gold nanoparticle-based detection strategy for ATP assays. In the absence of ATP, gold nanoparticles are not stabilized by the rigid duplex, thus they are readily aggregated by salt (solution displaying blue colors); in contrast induces a duplex-to-aptamer structural switching, liberating a random coil-like ssDNA. Gold nanoparticles are stabilized by the liberated ssDNA, showing high resistance to salt-induced aggregation (solution staying in red).

引用

收藏

页码：3943 / 3946

页数：4

相关论文

共 26 条

[11] In vitro selection of signaling aptamers [J].

Jhaveri, S ;

Rajendran, M ;

Ellington, AD .

NATURE BIOTECHNOLOGY, 2000, 18 (12) :1293-1297

[12] Designed signaling aptamers that transduce molecular recognition to changes in fluorescence intensity [J].

Jhaveri, SD ;

Kirby, R ;

Conrad, R ;

Maglott, EJ ;

Bowser, M ;

Kennedy, RT ;

Glick, G ;

Ellington, AD .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (11) :2469-2473

[13] Colorimetric detection of DNA sequences based on electrostatic interactions with unmodified gold nanoparticles [J].

Li, HX ;

Rothberg, L .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (39) :14036-14039

[14] Label-free colorimetric detection of specific sequences in genomic DNA amplified by the polymerase chain reaction [J].

Li, HX ;

Rothberg, LJ .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (35) :10958-10961

[15] Structural basis of DNA folding and recognition in an AMP-DNA aptamer complex: distinct architectures but common recognition motifs for DNA and RNA aptamers complexed to AMP [J].

Lin, CH ;

Patel, DJ .

CHEMISTRY & BIOLOGY, 1997, 4 (11) :817-832

[16] Proofreading and error removal in a nanomaterial assembly [J].

Liu, JW ;

Wernette, DP ;

Lu, Y .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2005, 44 (44) :7290-7293

[17] Structure-switching signaling aptamers [J].

Nutiu, R ;

Li, YF .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (16) :4771-4778

[18] Structure-switching signaling aptamers: Transducing molecular recognition into fluorescence signaling [J].

Nutiu, R ;

Li, YF .

CHEMISTRY-A EUROPEAN JOURNAL, 2004, 10 (08) :1868-1876

[19] Aptamer-functionalized Au nanoparticles for the amplified optical detection of thrombin [J].

Pavlov, V ;

Xiao, Y ;

Shlyahovsky, B ;

Willner, I .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (38) :11768-11769

[20] Nanostructures in biodiagnostics [J].

Rosi, NL ;

Mirkin, CA .

CHEMICAL REVIEWS, 2005, 105 (04) :1547-1562