Allosterically activated Diels-Alder catalysis by a ribozyme

被引：31

作者：

Helm, M ^{[1
]}

Petermeier, M ^{[1
]}

Ge, BX ^{[1
]}

Fiammengo, R ^{[1
]}

Jäschke, A ^{[1
]}

机构：

[1] Univ Heidelberg, Inst Pharm & Mol Biotechnol, D-69120 Heidelberg, Germany

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2005年 / 127卷 / 30期

关键词：

D O I：

10.1021/ja052886i

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We describe the allosteric control of Diels-Alder reactions by a small organic effector, theophylline. This is achieved by converting a Diels-Alder ribozyme into an allosterically regulated system. In contrast to other published systems, we have a bond-forming reaction with two small-molecule substrates and multiple turnover. This system could be very attractive for the development of assays for a variety of analytes and can be regarded as a prototype of fully synthetic signaling cascades. Copyright © 2005 American Chemical Society.

引用

页码：10492 / 10493

页数：2

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