Consequence of boar edible tissue consumption on urinary profiles of nandrolone metabolites.: II.: Identification and quantification of 19-norsteroids responsible for 19-norandrosterone and 19-noretiocholanolone excretion in human urine

In previous work (Le Bizec et al., Rapid Commun. Mass Spectrom. 2000; 14:1058), it was demonstrated that a boar meal intake could lead to possible false accusations of abuse of 17 beta -nortestosterone in antidoping control. The aim of the present study was to identify and quantify endogenous 19-norsteroids in boar edible tissue at concentrations that can alter the steroid urinary profile in humans, and lead to excretion of 19-norandrosterone (19-NA) and 19-noretiocholanolone (19-NE). The samples were analysed in two laboratories. The methodologies used for extraction and detection (GC/MS(EI) and LC/MS/MS(APCI+)) are compared and discussed. 19-Norandrostenedione (NAED), 17 beta- and 17 alpha -nortestosterone (bNT, aNT), and 17 beta- and 17 alpha -testosterone (bT, aT) were quantified. The largest concentrations of NAED and bNT were observed in testicles (83 and 172 mug/kg), liver (17 and 63 mug/kg) and kidney (45 and 38 mug/kg). A correlation between the bNT and NAED content of a typical meal prepared with boar parts and the excreted concentrations of 19-NA and 19-NE in human urine was demonstrated. Copyright (C) 2001 John Wiley & Sons, Ltd.