Studies toward the site specific incorporation of sugars into proteins: Synthesis of glycosylated aminoacyl-tRNAs

被引：15

作者：

Fahmi, NE

Golovine, S

Wang, BX

Hecht, SM

机构：

[1] Univ Virginia, Dept Chem, Charlottesville, VA 22901 USA

[2] Univ Virginia, Dept Biol, Charlottesville, VA 22901 USA

来源：

CARBOHYDRATE RESEARCH | 2001年 / 330卷 / 02期

关键词：

glycoprotein; monosaccharide; S-serine; site-specific glycosylation; transfer RNA;

D O I：

10.1016/S0008-6215(00)00289-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A series of glycosylated serine derivatives was synthesized from peracetylated sugars and Fmoc-protected serine; these were chemically esterified with the tris-(tetrabutylammoniurn) salt of pdCpA. The fully protected and deprotected glycosylated aminoacyl pdCpAs were ligated enzymatically to an abbreviated tRNA (tRNA-C-OH) to provide the title compounds that are key intermediates in the elaboration of glycoproteins using readthrough of a nonsense codon. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：149 / 164

页数：16

共 79 条

[1]

ALPIN JD, 1981, CRC CRIT REV BIOCH, V10, P259

[2] EFFECTS OF GLYCOSYLATION ON PEPTIDE BACKBONE CONFORMATION [J].