Tuberculosis drug resistance testing by molecular methods: Opportunities and challenges in resource limited settings

被引：24

作者：

Abebe, Gemeda ^{[1
,5
]}

Paasch, Fabienne ^{[2
]}

Apers, Ludwig ^{[3
]}

Rigouts, Leen ^{[4
]}

Colebunders, Robert ^{[3
,5
]}

机构：

[1] Jimma Univ, Dept Med Lab Sci & Pathol, Jimma, Ethiopia

[2] Univ Antwerp, Vaccine & Infect Dis Inst, Dept Med Microbiol, B-2020 Antwerp, Belgium

[3] Inst Trop Med Prince Leopold, Dept Clin Sci, B-2000 Antwerp, Belgium

[4] Inst Trop Med Prince Leopold, Dept Microbiol, Mycobacteriol Unit, B-2000 Antwerp, Belgium

[5] Univ Antwerp, Dept Epidemiol & Social Med, B-2020 Antwerp, Belgium

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2011年 / 84卷 / 02期

关键词：

Tuberculosis; Drug-resistance; Resource-limited settings; STRAND CONFORMATION POLYMORPHISM; RESONANCE ENERGY-TRANSFER; POLYMERASE-CHAIN-REACTION; GENOTYPE MTBDR ASSAY; NEW-YORK-CITY; MYCOBACTERIUM-TUBERCULOSIS; RAPID DETECTION; RIFAMPIN RESISTANCE; CLINICAL SPECIMENS; SPECIES IDENTIFICATION;

D O I：

10.1016/j.mimet.2010.11.014

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

One of the greatest threats to global tuberculosis (TB) control is the growing prevalence of drug-resistant bacilli. Correctly diagnosing drug-resistant TB patients is more problematic in resource-limited settings as there is no or limited infrastructure for drug susceptibility testing (DST) of TB bacilli. The conventional phenotypic DST method for TB takes weeks before declaring the results and initiating proper anti-TB treatment. Molecular DST offers advantages over the phenotypic methods mainly because of the short turnaround time. This review summarizes the different molecular DST methods for TB and discusses challenges and opportunities in implementing them in resource-limited settings. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：155 / 160

页数：6

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