1-Ethynylpyrene-modified guanine and cytosine as optical labels for DNA hybridization

被引：45

作者：

Wagner, C ^{[1
]}

Rist, M ^{[1
]}

Mayer-Enthart, E ^{[1
]}

Wagenknecht, HA ^{[1
]}

机构：

[1] Tech Univ Munich, Dept Chem, D-85747 Garching, Germany

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2005年 / 3卷 / 11期

关键词：

D O I：

10.1039/b504079e

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

1-Ethynylpyrene shows remarkable absorption changes upon DNA hybridization when it is covalently attached to the 8-position of guanine. An absorption band at similar to 420 nm is only present in the duplex, exhibits thermal melting behaviour and provides the basis for a molecular beacon together with 1-ethynylpyrene-modified cytosine.

引用

页码：2062 / 2063

页数：2