Localization of gamma-aminobutyric acid and glutamic acid decarboxylase in the pancreas of the nonobese diabetic mouse

被引:26
作者
SaraviaFernandez, F
Faveeuw, C
BlasquezBulant, C
Tappaz, M
Throsby, M
Pelletier, G
Vaudry, H
Dardenne, M
HomoDelarche, F
机构
[1] UNIV PARIS 05, HOP NECKER, CNRS, URA 1461, F-75743 PARIS 15, FRANCE
[2] INST BIOL & MED EXPTL, RA-1428 BUENOS AIRES, DF, ARGENTINA
[3] UNIV ROUEN, INSERM, U413, F-76821 MONT ST AIGNAN, FRANCE
[4] INSERM, U171, F-69230 ST GENIS LAVAL, FRANCE
[5] UNIV LAVAL, CTR HOSP, MED RES CTR GRP MOL ENDOCRINOL, LAVAL, PQ GIV 4G2, CANADA
关键词
D O I
10.1210/en.137.8.3497
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Glutamic acid decarboxylase (GAD), among other potential autoantigens, is thought to play a crucial role in type I diabetes, particularly in a spontaneous model of the disease, the nonobese diabetic (NOD) mouse. In the pancreas, the presence of GAD and gamma-aminobutyric acid (GABA), the decarboxylation product of GAD and a putative neurotransmitter in the islets of Langerhans, is well documented in the beta-cells. This is particularly true in rats, in which another GABAergic structure exists near the islets, the neuronal bodies. In this study, first the GABA content was measured in isolated islets from NOD and C57BL/6 mice (controls), and a decrease was found in NOD females as their insulitis progressed. Second, for the first time in mice, confocal analysis of immunofluorescent-labeled pancreatic sections revealed near the islets neuronal structures in which GAD and neuropeptide Y were colocalized, as they are in the brain. These structures were always observed in the pancreata of both sexes of C57BL/6 mice at the various ages investigated. In NOD mice, however, these neuronal structures were only detected in young females (<10 weeks old) and in males until an intermediate age. Moreover, patches of T cells surrounding GAD-containing fibers were seen in the vicinity of the islets with incipient periinsulitis.
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页码:3497 / 3506
页数:10
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