Retinal flow cytometer

被引：23

作者：

Alt, C. ^{[1
,2
,3
]}

Veilleux, I. ^{[1
,2
]}

Lee, H. ^{[1
,2
,4
]}

Pitsillides, C. M. ^{[1
,2
,5
]}

Cote, D. ^{[1
,2
]}

Lin, C. P. ^{[1
,2
]}

机构：

[1] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Adv Microscopy Program,Ctr Syst Biol, Boston, MA 02114 USA

[2] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Wellman Ctr Photomed, Boston, MA 02114 USA

[3] Tufts Univ, Dept Biomed Engn, Medford, MA 02155 USA

[4] Kyungpook Natl Univ, Taegu 702701, South Korea

[5] Boston Univ, Dept Biomed Engn, Boston, MA 02215 USA

来源：

OPTICS LETTERS | 2007年 / 32卷 / 23期

关键词：

D O I：

10.1364/OL.32.003450

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

The in vivo flow cytometer is an instrument capable of continuous, real-time monitoring of fluorescently labeled cells in the circulation without the need to draw blood samples. However, the original system probes a single vessel in the mouse ear; the small sample volume limits the sensitivity of the technique. We describe an in vivo retinal flow cytometer that simultaneously probes five artery-vein pairs in the mouse eye by circularly scanning a small laser spot rapidly around the optic nerve head. We demonstrate that the retinal flow cytometer detects about five times more cells per minute than the original in vivo flow cytometer does in the ear. (c) 2007 Optical Society of America.

引用

页码：3450 / 3452

页数：3

共 8 条

[1]

Al-Shabrawey M, 2003, MOL VIS, V9, P549

[2] Portable two-color in vivo flow cytometer for real-time detection of fluorescently-labeled circulating cells [J].