Molecular inventory of faecal microflora in patients with Crohn's disease

被引:114
作者
Mangin, I
Bonnet, R
Seksik, P
Rigottier-Gois, L
Sutren, M
Bouhnik, Y
Neut, C
Collins, MD
Colombel, JF
Marteau, P
Doré, J
机构
[1] INRA, Unite Ecol & Physiol Syst Digest, F-78532 Jouy En Josas, France
[2] Univ Reading, Sch Food Biosci, Reading RG6 6AP, Berks, England
[3] Hop Europeen Georges Pompidou, Dept Hepato Gastroenterol, F-75908 Paris 15, France
[4] Hop Lariboisiere, Serv Hepato Gastroenterol & Assistance Nutrit, F-75475 Paris 10, France
[5] Fac Pharm, Bacteriol Lab, F-59006 Lille, France
[6] CHU Lille, Hop Huriez, Serv Hepato Gastroenterol, F-59037 Lille, France
关键词
Crohn's disease; 16S rRNA gene sequencing; faecal bacterial diversity;
D O I
10.1016/j.femsec.2004.05.005
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Intestinal microbial community is involved in the pathogenesis of Crohn's disease, but knowledge of its potential abnormalities has been limited by the impossibility to grow many dominant intestinal bacteria. Using sequence analysis of randomly cloned bacterial 16S ribosomal DNA, the dominant faecal species from four Crolin's disease patients and four controls were compared. Whereas marked inter-individual differences were observed in the faecal microflora of patients, three remained distantly related to controls on the basis of their operational taxonomic unit composition. Bacteroides vidgatus and closely related organisms represented the only molecular species shared by all patients and exhibited an unusually high rate of occurrence. Escherichia coli clones were isolated only in two patients with ileocolonic Crohn's disease. Moreover, numerous clones belonged to phylogenetic groups or species that are commonly not dominant in the faecal microflora of healthy subjects: Pectinatus, Sutterella, Verritcomicrobium, Fusobacterium, Clostridium disporicum, clostridium glycolicum, Clostridium ramosum, Clostridium innocuum and Clostridium perfringens. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码:25 / 36
页数:12
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