Concurrent fast and slow cycling of a transcriptional activator at an endogenous promoter

被引：114

作者：

Karpova, Tatiana S. ^{[1
]}

Kim, Min J. ^{[1
]}

Spriet, Corentin ^{[2
]}

Nalley, Kip ^{[1
]}

Stasevich, Timothy J. ^{[1
]}

Kherrouche, Zoulika ^{[3
]}

Heliot, Laurent ^{[2
]}

McNally, James G. ^{[1
]}

机构：

[1] NCI, Ctr Canc Res, Core Imaging Facil, Lab Receptor Biol & Gene Express,NIH, Bethesda, MD 20892 USA

[2] Lille Univ Sci & Technol, Interdisciplinary Res Inst, CNRS, USR 3078, F-59021 Lille, France

[3] Inst Pasteur, UMR 8161, Inst Biol Lille, F-59021 Lille, France

来源：

SCIENCE | 2008年 / 319卷 / 5862期

关键词：

D O I：

10.1126/science.1150559

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

For gene regulation, some transcriptional activators bind periodically to promoters with either a fast (similar to 1 minute) or a slow (similar to 15 to 90 minutes) cycle. It is uncertain whether the fast cycle occurs on natural promoters, and the function of either cycle in transcription remains unclear. We report that fast and slow cycling can occur simultaneously on an endogenous yeast promoter and that slow cycling in this system reflects an oscillation in the fraction of accessible promoters rather than the recruitment and release of stably bound transcriptional activators. This observation, combined with single- cell measurements of messenger RNA ( mRNA) production, argues that fast cycling initiates transcription and that slow cycling regulates the quantity of mRNA produced. These findings counter the prevailing view that slow cycling initiates transcription.

引用

页码：466 / 469

页数：4

共 22 条

[1] A Rsc3/Rsc30 zinc cluster dimer reveals novel roles for the chromatin remodeler RSC in gene expression and cell cycle control [J].