Accelerated wound closure in neutrophil-depleted mice

被引:405
作者
Dovi, JV
He, LK
DiPietro, LA
机构
[1] Loyola Univ, Ctr Med, Dept Microbiol & Immunol, Maywood, IL 60153 USA
[2] Loyola Univ, Ctr Med, Dept Surg, Burn & Shock Trauma Inst, Maywood, IL 60153 USA
关键词
healing; keratinocytes; inflammation; diabetic;
D O I
10.1189/jlb.0802406
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The infiltration of neutrophils into injured tissue is known to protect wounds from invading pathogens. However, more recent studies suggest that neutrophils might inhibit the wound repair process. To investigate the role of neutrophils in wounds, mice were nentrophil-depleted by injection with rabbit anti-mouse neutrophil serum. Remarkably, epidermal healing, measured by wound closure, proceeded significantly faster in neutropenic than control mice (77.7 + 14.2% vs. 41.2 + 0.9%, P < 0.02 at day 2). Dermal healing was not affected by neutrophil depletion, as neither collagen deposition nor wound-breaking strength was significantly different between neutropenic and control mice. As the delayed repair of diabetic individuals exhibits robust inflammation, the effect of neutrophil depletion on diabetic wound healing was investigated. Similar to the observations in wild-type mice, wound closure was accelerated by nearly 50% in neutropenic, diabetic mice. The results suggest that although neutrophils may provide protection against infection, they may retard wound closure. J. Leukoc. Biol. 73: 448-455; 2003.
引用
收藏
页码:448 / 455
页数:8
相关论文
共 26 条
[21]   Bioactive interleukin-8 is expressed in wounds and enhances wound healing [J].
Rennekampff, HO ;
Hansbrough, JF ;
Kiessig, V ;
Doré, C ;
Sticherling, M ;
Schröder, JM .
JOURNAL OF SURGICAL RESEARCH, 2000, 93 (01) :41-54
[22]   IMPROVED METHOD FOR QUANTIFICATION OF TISSUE PMN ACCUMULATION MEASURED BY MYELOPEROXIDASE ACTIVITY [J].
SCHIERWAGEN, C ;
BYLUNDFELLENIUS, AC ;
LUNDBERG, C .
JOURNAL OF PHARMACOLOGICAL METHODS, 1990, 23 (03) :179-186
[23]   NEUTROPHILIC LEUKOCYTE IN WOUND REPAIR - STUDY WITH ANTINEUTROPHIL SERUM [J].
SIMPSON, DM ;
ROSS, R .
JOURNAL OF CLINICAL INVESTIGATION, 1972, 51 (08) :2009-+
[24]   ASSAY-METHOD FOR MYELOPEROXIDASE IN HUMAN POLYMORPHONUCLEAR LEUKOCYTES [J].
SUZUKI, K ;
OTA, H ;
SASAGAWA, S ;
SAKATANI, T ;
FUJIKURA, T .
ANALYTICAL BIOCHEMISTRY, 1983, 132 (02) :345-352
[25]   Large and sustained induction of chemokines during impaired wound healing in the genetically diabetic mouse:: Prolonged persistence of neutrophils and macrophages during the late phase of repair [J].
Wetzler, C ;
Kämpfer, H ;
Stallmeyer, B ;
Pfeilschifter, J ;
Frank, S .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2000, 115 (02) :245-253